Quantitative cDNA-AFLP reveals the extent of transcriptional polymorphism in developing cotton fibres.

Claverie Michel, Souquet Marlène, Jean Janine, Forestier-Chiron Nelly, Lepitre Vincent, Pré Martial, Viot Christopher, Jacobs John, Llewellyn Danny, Lacape Jean-Marc. 2010. Quantitative cDNA-AFLP reveals the extent of transcriptional polymorphism in developing cotton fibres.. In : International Cotton Genome Initiative research conference ( ICGI 2010), Canberra, Australia, 20-23 September 2010. s.l. : s.n., Résumé, 84. International Cotton Genome Initiative Research Conference, Canberra, Australie, 20 Septembre 2010/23 Septembre 2010.

Résumé : Genetic variability in fibre quality among the two major cultivated cotton species, Gossypium hirsutum (Gh) and G. barbadense (Gb), shows a complex multigenic inheritance. We developed a quantitative 3 targeting cDNA-AFLP analysis strategy in order to dissect transcriptional regulation differences between the developing fibres of these 2 species. Two studies were undertaken. In the first study the expression profiles of over 3000 transcripts from the 2 parental species were analysed by quantitative cDNA-AFLP during the time-course of fibre development, between 6 and 28 days post anthesis (dpa). The 2nd study (4400 transcripts profiled) focused on two key developmental stages of fibre development (10 and 22 dpa, respectively) at a population-wide level using an inter-specific RIL population. Major achievements include: (1) the partitioning of genes among significant expression profiles and its comparison between Gh and Gb; and (2) the mapping of a large (>5000) number of expression QTLs on the RIL genetic map and a comparison of their distributions with QTL for fibre phenotypic traits. This research is part of a larger project aimed at the genetic and genomic dissection of cotton fibre quality.

Classification Agris : F30 - Génétique et amélioration des plantes
Q60 - Traitement des produits agricoles non alimentaires

Auteurs et affiliations

• Claverie Michel, CIRAD-BIOS-UMR DAP (FRA)
• Souquet Marlène, CIRAD-BIOS-UMR DAP (FRA)
• Jean Janine, CIRAD-PERSYST-UPR SCA (FRA) ORCID: 0009-0006-5220-0882
• Forestier-Chiron Nelly, CIRAD-PERSYST-UMR Qualisud (FRA)
• Lepitre Vincent, CIRAD-BIOS-UMR PVBMT (REU)
• Pré Martial, CIRAD-BIOS-UMR DAP (FRA)
• Viot Christopher, CIRAD-BIOS-UMR DAP (FRA) ORCID: 0000-0001-6321-4741
• Jacobs John, Bayer (BEL)
• Llewellyn Danny, CSIRO (AUS)
• Lacape Jean-Marc, CIRAD-BIOS-UMR DAP (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/565484/)

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