European ring trial to evaluate ELISAs for the diagnosis of infection with Rift Valley fever virus

Kortekaas Jeroen, Kant Jet, Vloet Rianka, Cetre-Sossah Catherine, Marianneau Philippe, Lacote Sandra, Banyard Ashley C., Jeffries Claire, Eiden Martin, Groschup Martin, Jäckel Susanne, Hevia Esther, Brun Alejandro. 2013. European ring trial to evaluate ELISAs for the diagnosis of infection with Rift Valley fever virus. Journal of Virological Methods, 187 (1) : 177-181.

Quartile : Q3, Sujet : BIOTECHNOLOGY & APPLIED MICROBIOLOGY / Quartile : Q3, Sujet : BIOCHEMICAL RESEARCH METHODS / Quartile : Q4, Sujet : VIROLOGY

Résumé : A ring trial was organized to evaluate Rift Valley fever virus (RVFV) ELISAs by European laboratories. A total of five ELISAs, two of which specific for IgM antibodies, were evaluated by six participants. Sera were derived from cattle or sheep and originated from either a RVFV endemic area, a RVFV-free area or from experimental infection studies. Cohen's kappa analysis showed higher than 90% agreement of two commercially available ELISAs with the virus neutralization test, suggesting that primary screening as well as serological confirmation using these ELISAs is feasible. More extensive validations with sera of known IgM status are, however, required to determine agreement between IgM ELISAs.

Mots-clés Agrovoc : Virus de la fièvre de la vallée du Rift, Test ELISA, immunologie, diagnostic, infection expérimentale, expérimentation en laboratoire, ovin, bovin

Mots-clés géographiques Agrovoc : Europe

Classification Agris : L73 - Maladies des animaux
U30 - Méthodes de recherche

Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes

Auteurs et affiliations

• Kortekaas Jeroen, Central Veterinary Institute (NLD)
• Kant Jet, Central Veterinary Institute (NLD)
• Vloet Rianka, Central Veterinary Institute (NLD)
• Cetre-Sossah Catherine, CIRAD-BIOS-UMR CMAEE (FRA)
• Marianneau Philippe, ANSES (FRA)
• Lacote Sandra, ANSES (FRA)
• Banyard Ashley C., Wildlife Zoonoses and Vector Borne Disease Research Group (GBR)
• Jeffries Claire, Wildlife Zoonoses and Vector Borne Disease Research Group (GBR)
• Eiden Martin, Federal Research Institute for Animal Health (DEU)
• Groschup Martin, Federal Research Institute for Animal Health (DEU)
• Jäckel Susanne, Federal Research Institute for Animal Health (DEU)
• Hevia Esther, CISA (ESP)
• Brun Alejandro, CISA (ESP)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/566671/)

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