A new minisatellite-based scheme for the global surveillance of Xanthomonas citri pv. citri, the causal agent of Asiatic Citrus Canker. : S12O12

Magne Maxime, Leduc Alice, Vernière Christian, Vital Karine, Gordon Jonathan, Escalon Aline, Guérin Fabien, Gagnevin Lionel, Pruvost Olivier. 2012. A new minisatellite-based scheme for the global surveillance of Xanthomonas citri pv. citri, the causal agent of Asiatic Citrus Canker. : S12O12. In : XII International Citrus Congress : Book of abstract. Navarro Luis (ed.). ISC, IVIA, Fundación agroalimed. Riverside : ISC, Résumé, 197-198. International Citrus congress "Citrus and health. 12, Valence, Espagne, 18 Novembre 2012/23 Novembre 2012.

Résumé : Xanthomonas citri pv. citri (Xcc), the causal agent of Asiatic Citrus Canker, is an internationally major bacterial pathogen currently not reported from countries in the Mediterranean basin, including the EU. Some pathological diversity among strains was reported, leading to the description in Xcc of pathotypes differing in host range and aggressiveness. Xcc is recognized as a monomorphic pathogen and the very low intrapathovar diversity at housekeeping genes precludes the use of the increasingly popular MLST (MultiLocus Sequence Typing) for the global molecular surveillance of Xcc. We developed a new MLVA (MultiLocus Variable number of tandem repeats Analysis) scheme. It targeted 51 minisatellite loci dispersed over the Xcc genome with tandem repeat size ranging from 10 to 217-bp, thus allowing the use of standard agarose gel electrophoresis in laboratories that are not equipped with a genotyper. Among these loci, 34 were found to be polymorphic within a strain collection composed of strains from all continents where Xcc has been recorded, including recent outbreaks in Africa where the bacterium is reemerging. This typing scheme displayed a maximal typeability and repeatability. The produced data support the usefulness of this new MLVA scheme for global surveillance of Xcc. (Texte integral)

Classification Agris : H20 - Maladies des plantes

Auteurs et affiliations

• Magne Maxime, CIRAD-BIOS-UMR PVBMT (REU)
• Leduc Alice
• Vernière Christian, CIRAD-BIOS-UMR PVBMT (REU) ORCID: 0000-0002-2312-2073
• Vital Karine, CIRAD-BIOS-UMR PVBMT (REU)
• Gordon Jonathan, Université de la Réunion (REU)
• Escalon Aline, CIRAD-BIOS-UMR PVBMT (REU)
• Guérin Fabien, Université de la Réunion (REU)
• Gagnevin Lionel, CIRAD-BIOS-UMR PVBMT (REU) ORCID: 0000-0002-2943-0827
• Pruvost Olivier, CIRAD-BIOS-UMR PVBMT (REU)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/567346/)

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