Xanthomonas albilineans needs an OmpA family outer protein for disease symptom development and multiplication in the sugarcane stalk : [Abstracts]

Rott Philippe, Fleites Laura, Marlow Gary C., Royer Monique, Gabriel Dean W.. 2010. Xanthomonas albilineans needs an OmpA family outer protein for disease symptom development and multiplication in the sugarcane stalk : [Abstracts]. Phytopathology, 100 (6S), suppl., Résumé, S111 2010 APS Annual Meeting, Charlotte, États-Unis, 7 Août 2010/11 Août 2010.

Quartile : Q1, Sujet : PLANT SCIENCES

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie

Résumé : Xanthomonas albilineans (Xa) is a systemic, xylem-invading pathogen that causes sugarcane leaf scald. Xa produces albicidin, a potent antibiotic and phytotoxin which blocks chloroplast differentiation, thus causing the foliar symptoms of the disease. Albicidin is the only known pathogenicity factor in Xa, yet albicidin deficient mutants are still able to colonize the sugarcane plant. In an attempt to identify other major pathogenicity factors, we screened 1,216 independent Tn5 insertions in Xa strain XaFL07-1 by single inoculation onto sugarcane cultivar CP80-1743. Mutants were screened for reduced pathogenicity (i.e., capacity to induce leaf symptoms and to multiply in the sugarcane stalk). Five independent Tn5 insertions were found in gene XALc_0557, which is predicted to encode an OmpA family outer membrane protein. Each of these insertions resulted in a mutant strain that elicited very slight to no symptoms and was not able to move as efficiently within the sugarcane stalk, both spatially and in intensity, as wild type XaFL07-1. Additional phenotypic studies showed that these mutants: 1) produced albicidin, 2) were less motile and 3) were slower growing than the wild type Xa in vitro. However, these OmpA mutants were able to multiply in sugarcane leaf tissue to levels similar to the wild-type strain XaFL07-1. Complementation analyses are currently underway. (Texte intégral)

Mots-clés Agrovoc : Xanthomonas albilineans, Saccharum officinarum, mutant, testage, pouvoir pathogène, protéine microbienne

Mots-clés complémentaires : Albicidine

Classification Agris : H20 - Maladies des plantes

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Rott Philippe, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0001-6085-6159
• Fleites Laura, University of Florida (USA)
• Marlow Gary C., University of Florida (USA)
• Royer Monique, CIRAD-BIOS-UMR BGPI (FRA)
• Gabriel Dean W., University of Florida (USA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/568040/)

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