microRNAs: new regulatores of biological function in Hevea brasilienis

Leclercq Julie, Gebelin Virginie, Kuswanhadi, Chaidamsari Tetty, Duan Cuifang, Montoro Pascal. 2012. microRNAs: new regulatores of biological function in Hevea brasilienis. In : IRC 2012 International Rubber Conference : Abstracts, Kovalam, India, 28-31 October 2012. s.l. : s.n., Résumé, 76-77. International Rubber Conference, Kovalam, Inde, 28 Octobre 2012/31 Octobre 2012.

Résumé : Fine regulation of gene expression is partially ensured by microRNAs (miRNAs) in response to external stimuli. They negatively regulate gene expression by targeting the cleavage or inhibit the translation of target messenger RN As (mRNAs). In Hevea brasiliensis, environmental and harvesting stresses are known to affect natural rubber production. Deep sequencing of small RN As was carried out on plantlets, subjected to severe abiotic stress, and on latex from trees affected or not by TPD, using the Solexa technique. By combining the LeARN pipeline, data from the Plant microRNA database (PMRD) and Hevea EST sequences, we identified 68 conserved miRNA families already characterized in other plant species, and 15 putatively novel miRNA families. MiRNA targets were computationally predicted and analysed. Targets involved in rubber biosynthesis, ROS-scavenging systems and ethylene perception and transduction pathway are presented.

Mots-clés Agrovoc : Hevea brasiliensis, régulation physiologique, arn, latex, stress, oxydation

Classification Agris : F60 - Physiologie et biochimie végétale
F30 - Génétique et amélioration des plantes
H50 - Troubles divers des plantes

Auteurs et affiliations

• Leclercq Julie, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-8110-3762
• Gebelin Virginie, CIRAD-BIOS-UMR AGAP (FRA)
• Kuswanhadi, IRRI [Indonesian Rubber Research Institute] (IDN)
• Chaidamsari Tetty, IBRIEC (IDN)
• Duan Cuifang, CATAS (CHN)
• Montoro Pascal, CIRAD-BIOS-UMR AGAP (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/568058/)

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