Comparison of accessions conserved in different litchi germplasm collections using microsatellite markers

Madhou Madhvee, Normand Frédéric, Bahorun Theeshan, Hormaza José Ignacio. 2014. Comparison of accessions conserved in different litchi germplasm collections using microsatellite markers. In : Proceedings of the 4th International Symposium on Lychee, Longan and Other Sapindaceae Fruits, White River, South Africa, 2-6 December, 2012. R. Cronje (ed.). Louvain : ISHS, Résumé, 93-99. (Acta Horticulturae, 1029) ISBN 978-94-62610-15-6 International Symposium on Lychee, Longan and Other Sapindaceae fruits. 4, White River, Afrique du Sud, 2 Décembre 2012/6 Décembre 2012.

Résumé : One of the main limitations for breeding purposes and optimum germplasm management of litchi is the confusion in cultivar denomination among different litchi producing countries and germplasm collections worldwide that results in a high number of homonymies and synonymies. In this work, we have characterized 88 litchi accessions conserved in three different germplasm collections (Mauritius, Réunion and Spain) using microsatellite markers. A total of Il simple sequence repeat (SSR) loci were used that yielded 67 amplification fragments, with an average of 6.1 amplification bands/SSR. Eight primer pairs amplified single loci in the accessions were examined. The mean expected and observed heterozygosiiies over those 8 single locus SSRs averaged 0.53 and 0.61, respectively, with a value for the probability of identity of 9.78 x 10"'- A total of 42 different genetic profiles were found among the 88 samples analyzed and several synonymies and homonymies both within and among the germplasm collections were found. No c1ear geographical trends could be observed among the groups obtained in the UPGMA dendrogram or with the STRUCTURE software. The results demonstrate the usefulness ofthis kind ofmolecular marker for the optimization oflitchi gem1plasm management and they will be discussed in terms of their implications for the standardization of litchi cultivar nomenclature over the studied germplasm collections and in other litchi producing countries in order to have a c1ear picture of current litchi germplasm conservation worldwide. (Texte intégral)

Classification Agris : F30 - Génétique et amélioration des plantes
F70 - Taxonomie végétale et phytogéographie

Auteurs et affiliations

• Madhou Madhvee, Mauritius research council (MUS)
• Normand Frédéric, CIRAD-PERSYST-UPR HortSys (REU) ORCID: 0000-0002-4322-8262
• Bahorun Theeshan, University of Mauritius (MUS)
• Hormaza José Ignacio, Universidad de Málaga (ESP)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/568079/)

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