Quantitative Trait Loci mapping for sugar-related traits in sweet sorghum based on high-density SNP markers : P0187

Pereira Guilherme S., Souza Vander Fillipe, Parrella Rafael Augusto C, Damasceno Cynthia Maria B, Simeone Maria Lucia F., Schaffert Robert E., Braconnier Serge, Garcia Antonio Augusto Franco, Magalhaes Jurandir V.. 2013. Quantitative Trait Loci mapping for sugar-related traits in sweet sorghum based on high-density SNP markers : P0187. In : Plant and Animal Genome XXI conference, San Diego, USA, January 12-16 2013,. s.l. : s.n., Résumé, 1 p. Plant and Animal Genome conference. 21, San Diego, États-Unis, 12 Janvier 2013/16 Janvier 2013.

Résumé : Sweet sorghum, a C4 tropical grass, is becoming an important bioenergy crop. Characterizing quantitative trait loci (QTL) controlling sugar content in its stem can lead to the identification of target regions for molecular breeding programs. These QTLs may be conserved in related species such as sugarcane, expanding their potential usefulness for bioenergy production. In this study, 223 recombinant inbred lines (RILs) derived from the sweet sorghum parents, Brandes and Wray, were genotyped-by-sequencing (GBS). A lattice experiment with three replicates was established for evaluating three sugar related traits: soluble solids content (BRIX, in °Brix), sucrose content (POL, in %), and reducing sugars (RSU, in %). Phenotypic analysis and QTL scanning were conducted using mixed and general linear models approaches, using the softwares GenStat and TASSEL, respectively. GBS provided wide genome coverage, with ~1200 single-nucleotide polymorphisms (SNPs) per chromosome. Major QTLs were detected for BRIX and POL on chromosome 3, and for RSU on chromosome 4, explaining up to 20.8%, 18.9% and 15.5% of the phenotypic variance. False discovery rate correction suggested that QTLs were co-located on chromosome 1 for all traits as well. The QTLs identified here will be further investigated for possible use in marker-assisted selection targeting biofuel production in sweet sorghum.

Classification Agris : F30 - Génétique et amélioration des plantes
F60 - Physiologie et biochimie végétale
U30 - Méthodes de recherche

Auteurs et affiliations

• Pereira Guilherme S., USP (BRA)
• Souza Vander Fillipe, Federal University of São João Del-Rei (BRA)
• Parrella Rafael Augusto C, EMBRAPA (BRA)
• Damasceno Cynthia Maria B, EMBRAPA (BRA)
• Simeone Maria Lucia F., EMBRAPA (BRA)
• Schaffert Robert E., EMBRAPA (BRA)
• Braconnier Serge, CIRAD-BIOS-UMR AGAP (FRA)
• Garcia Antonio Augusto Franco, USP (BRA)
• Magalhaes Jurandir V., EMBRAPA (BRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/568345/)

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