Monitoring the banana response to infection by Mycosphaerella fijiensis : N76

Carreel Françoise, Da Silva Corinne, Labadie Karine, Souquet Marlène, Habas Rémy, Pignolet Luc, Zapater Marie-Françoise, Yahiaoui Nabila. 2013. Monitoring the banana response to infection by Mycosphaerella fijiensis : N76. In : International conference "Plant Diseases and Resistance Mechanisms" programme and abstracts, Vienna, Austria, February 20-22th 2013. Vienna International Plant Conference Association (VIPCA). Vienna : VIPCA, Résumé, 73. International Conference "Plant Diseases and Resistance Mechanisms", Vienne, Autriche, 20 Février 2013/22 Février 2013.

Résumé : Mycosphaerella fijiensis is a hemibiotrophic ascomycete causing the black leaf streak disease (BLSD) of banana that is commonly called Black Sigatoka disease. BLSD is regarded as the most economically important threat to banana production and affects almost all edible banana cultivars grown worldwide. The M. fijiensis infection cycle goes through a long symptomless phase in the plant, during which the fungus grows in the mesophyll intercellular space, before the appearance of disease symptoms in the form of necrotic areas. Despite the economical importance of the BLSD disease, little is known about the physiological and molecular events occurring during the pathogen life cycle in the plant and on the genetic bases of resistance. To better characterize the interaction, we first established a bioassay based on detached leaves maintained in vitro, associated to phenotyping with an image analysis software package (Visilog® Noesis). This allows higher throughput phenotyping and more precise monitoring of the reaction of banana accessions to M. fijiensis strains. In a preliminary experiment, we also analysed transcriptome changes in three accessions showing contrasted reactions to M. fijiensis, using RNA-Seq. First results of this analysis will be presented. (Texte intégral)

Classification Agris : H20 - Maladies des plantes
U30 - Méthodes de recherche

Auteurs et affiliations

• Carreel Françoise, CIRAD-BIOS-UMR AGAP (FRA)
• Da Silva Corinne, Institut de génomique (FRA)
• Labadie Karine, Institut de génomique (FRA)
• Souquet Marlène
• Habas Rémy, CIRAD-BIOS-UMR BGPI (FRA)
• Pignolet Luc, CIRAD-BIOS-UMR BGPI (FRA)
• Zapater Marie-Françoise, CIRAD-BIOS-UMR BGPI (FRA)
• Yahiaoui Nabila, CIRAD-BIOS-UMR AGAP (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/568648/)

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