Bovine respiratory syncytial virus: immunohistochemichal detection in mouse and bovine tissues using a Mab against human respiratory syncytial virus

Servan de Almeida Renata, Spilki Fernando Rosado, Roehe Paulo Michel, Verinaud Liana M.C., Arns Clarice Weis. 2006. Bovine respiratory syncytial virus: immunohistochemichal detection in mouse and bovine tissues using a Mab against human respiratory syncytial virus. Brazilian Journal of Veterinary Research and Animal Science, 58 (6) : 973-981.

Titre portugais : Vírus respiratório sincicial bovino: detecção por imunoistoquímica em tecidos de camundongos

Résumé : An immunoistochemical (IHC) test was developed to detect bovine respiratory syncytial virus (BRSV) in cell cultures and tissues of experimentally infected mice and calves, using a commercial monoclonal antibody (Mab) against human respiratory syncytial virus (HRSV), as a less expensive alternative, instead of producing specific monoclonal antibodies to BRSV. Clinical samples from calves suffering respiratory disease were also submitted to this test. IHC detected BRSV antigens in mouse tracheas (3, 5 and 7 days post-infection) and lungs (5 and 7 days post-infection), and in one of three lungs from experimentally infected calves. Lungs samples from two naturally infected calves were tested and resulted positive for BRSV by the IHC test. These results suggest that this test may be used in the future for diagnosis as well as a useful tool to assess the distribution of BRSV infections in Brazilian herds.

Mots-clés géographiques Agrovoc : Brésil

Classification Agris : L73 - Maladies des animaux
S50 - Santé humaine

Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes

Auteurs et affiliations

• Servan de Almeida Renata, CIRAD-EMVT-UPR Contrôle des maladies (FRA)
• Spilki Fernando Rosado, UNICAMP (BRA)
• Roehe Paulo Michel, Institute of Basic Health Sciences (BRA)
• Verinaud Liana M.C., UNICAMP (BRA)
• Arns Clarice Weis, UNICAMP (BRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/568794/)

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