d'Arce Regina Celia Freitas, Coswig Lia T., Servan de Almeida Renata, Trevisol Iara M., Monteiro Maria C.B., Rossini Lavínia I., Di Fabio José, Hafez Hafez M., Arns Clarice Weis. 2005. Subtyping of new Brazilian avian metapneumovirus isolates from chickens and turkeys by reverse transcriptase-nested-polymerase chain reaction. Avian Pathology, 34 (2) : 133-136.
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Résumé : The aim of this study was to improve a reverse transcriptase (RT)-nested-polymerase chain reaction (PCR) able to differentiate avian pneumovirus (APV) subtypes A and B, and to characterize new Brazilian isolates. Representative APV strains and clinical field samples from chickens and turkey flocks were amplified in the chicken embryo-related cell line. Viral RNA was extracted from harvested cells, and submitted to cDNA synthesis. The primers utilized for RT-PCR were compatible with the G gene of both the A and B subtypes of APV, while the nested primers were subtype specific. This approach showed that three new APVs from chickens and one from turkeys were subtype A, confirmed by sequencing. This is the first report of APV isolation from turkeys in Brazil. Four other APVs were detected and classified as subtype A by RT-nested- PCR. These optimized techniques could be useful for differentiation of APV subtypes A and B, proving to be a valuable molecular epidemiological tool.
Mots-clés géographiques Agrovoc : Brésil
Classification Agris : L73 - Maladies des animaux
Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes
Auteurs et affiliations
- d'Arce Regina Celia Freitas, UNICAMP (BRA)
- Coswig Lia T., UNICAMP (BRA)
- Servan de Almeida Renata, CIRAD-EMVT-UPR Contrôle des maladies (FRA)
- Trevisol Iara M., Sadia (BRA)
- Monteiro Maria C.B., JF Laboratory of Animal Pathology (BRA)
- Rossini Lavínia I., JF Laboratory of Animal Pathology (BRA)
- Di Fabio José, JF Laboratory of Animal Pathology (BRA)
- Hafez Hafez M., Free University Berlin (DEU)
- Arns Clarice Weis, UNICAMP (BRA)
Source : Cirad - Agritrop (https://agritrop.cirad.fr/568800/)
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