Zhe Li, Xuemei Dai, Aihua Sun, Tiandai Huang, Quannan Zhou, Huasun Huang, Weifu Lin, Lardet Ludovic, Montoro Pascal, Carron Marc-Philippe. 2010. Nucellus friable callus induction and its embryoid ontogenyfrom Rubber tree clone Reyan88-13 Hevea brasiliensis Müll. Arg.). Journal of Tropical Organisms, 1 (4) : 308-314.
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- Chinois
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Titre chinois : En chinois
Résumé : The nucellar tissue from the young seeds of rubber tree clone Reyan88-13 was used to induce embryogenic callus. The different culture parameters such as the different types and concentrations of plant growth regulators and different calcium concentrations had been studied in embryogenic caHus induction media and subculture media. The proper culture parameters had been determined. Via continuous subculture and selection during 5 months, friable and embryogenic callus had been gradually obtained. Long-term subculture had been carried on for the friable and embryogenic calli. Histological sections proved that the calli proliferated by long-term subculture maintained the embryogenic characters. The nucellar friable and embryogenic calli of rubber tree clone Reyan88-13 that had been subcultured for more than 2 years were used for inducing embryoids. At present 186 embryoids were obtained. These embryoids were being used to induce plant regeneration.
Mots-clés Agrovoc : Hevea brasiliensis, développement biologique, Embryogénèse somatique, milieu de culture, culture d'embryon, clone, régénération in vitro
Mots-clés géographiques Agrovoc : Chine
Classification Agris : F02 - Multiplication végétative des plantes
F62 - Physiologie végétale - Croissance et développement
Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique
Auteurs et affiliations
- Zhe Li, CATAS (CHN)
- Xuemei Dai, CATAS (CHN)
- Aihua Sun, CATAS (CHN)
- Tiandai Huang, CATAS (CHN)
- Quannan Zhou, CATAS (CHN)
- Huasun Huang, CATAS (CHN)
- Weifu Lin, CATAS (CHN)
- Lardet Ludovic, CIRAD-BIOS-UMR DAP (FRA)
- Montoro Pascal, CIRAD-BIOS-UMR DAP (FRA)
- Carron Marc-Philippe, CIRAD-BIOS-UMR DAP (FRA)
Source : Cirad - Agritrop (https://agritrop.cirad.fr/569141/)
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