Lipid transfer proteins in coffee: isolation of a Coffea orthologs, Coffea arabica homeologs, expression during coffee fruit development and promoter analysis in transgenic tobacco plants

Cotta Michelle G., Barros Leila M.G., De Almeida Juliana D., Santana R.H., Barbosa Eder A., Alves Gabriel Sergio Costa, Paiva L.V., Carneiro Mauro, Andrade Alan Carvalho, Marraccini Pierre. 2013. Lipid transfer proteins in coffee: isolation of a Coffea orthologs, Coffea arabica homeologs, expression during coffee fruit development and promoter analysis in transgenic tobacco plants. In : Proceedings of the 24th International Conference on Coffee Science, San José, Costa Rica, 12th-16th November 2012 = Actes du 24ème Colloque scientifique international sur le café, San José, Costa Rica, 12-16 novembre 2012. ASIC. Paris : ASIC, 1455-1459. ISBN 978-2-900212-23-3 International Conference on Coffee Science. 24, San José, Costa Rica, 12 Novembre 2012/16 Novembre 2012.

Résumé : Our study aims (i) to identify different coffee nsLTPs gene homeologs corresponding to the Coffea arabica (Ca) ancestor sub-genomes: C. canephora (Cc) and C. eugenioides (Ce); (ii) to evaluate the expression of these genes during the bean development and (iii) to characterize a grain-specific nsLTP promoter. We cloned three nsLTP-encoding cDNA highly expressed in coffee fruits. By RT-qPCR assays, the expression of each nsLTP genes carried by the Cc and Ce sub-genomes of Ca was also analyzed. The results highlighted the preferential expression of these genes in endosperm at 120 and 180 days after flowering (DAF). A nsLTP promoter region (1.2 kb) was also isolated and analyzed by 5' deletions for the ability to control the expression of the uidA reporter gene in transgenic Nicotiana tabacum plants. The GUS histochemical assays showed that 1.2 kb, 1.0 kb and 0.8 kb fragments of the nsLTP promoter directed the uidA expression to all tested plant organs with different activity levels while low or null expression was detected in roots. However, the 0.4kb fragment led to the expression of the uidA gene only in seeds, fruit and floral buds.

Classification Agris : F30 - Génétique et amélioration des plantes
F60 - Physiologie et biochimie végétale

Auteurs et affiliations

• Cotta Michelle G., UFLA (BRA)
• Barros Leila M.G., EMBRAPA (BRA)
• De Almeida Juliana D., EMBRAPA (BRA)
• Santana R.H., UNB [Universidade de Brasilia] (BRA)
• Barbosa Eder A., EMBRAPA (BRA)
• Alves Gabriel Sergio Costa, EMBRAPA (BRA)
• Paiva L.V., UFLA (BRA)
• Carneiro Mauro, EMBRAPA (BRA)
• Andrade Alan Carvalho, EMBRAPA (BRA)
• Marraccini Pierre, CIRAD-BIOS-UMR AGAP (BRA) ORCID: 0000-0001-7637-6811

Source : Cirad - Agritrop (https://agritrop.cirad.fr/569403/)

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