Genetically based location from triploid populations and gene ontology of a 3.3-Mb genome region linked to alternaria brown spot resistance in citrus reveal clusters of resistance genes

Cuenca José, Aleza Pablo, Vicent Antonio, Brunel Dominique, Ollitrault Patrick, Navarro Luis. 2013. Genetically based location from triploid populations and gene ontology of a 3.3-Mb genome region linked to alternaria brown spot resistance in citrus reveal clusters of resistance genes. PloS One, 8 (10):e76755, 18 p.

https://doi.org/10.1371/journal.pone.0076755

Article de revue ; Article de revue à facteur d'impact Revue en libre accès total

Prévisualisation

Version publiée - Anglais
Utilisation soumise à autorisation de l'auteur ou du Cirad.
document_570806.pdf
Télécharger (2MB) | Prévisualisation

Quartile : Outlier, Sujet : MULTIDISCIPLINARY SCIENCES

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie; Staps

Résumé : Genetic analysis of phenotypical traits and marker-trait association in polyploid species is generally considered as a challenge. In the present work, different approaches were combined taking advantage of the particular genetic structures of 2n gametes resulting from second division restitution (SDR) to map a genome region linked to Alternaria brown spot (ABS) resistance in triploid citrus progeny. ABS in citrus is a serious disease caused by the tangerine pathotype of the fungus Alternaria alternata. This pathogen produces ACT-toxin, which induces necrotic lesions on fruit and young leaves, defoliation and fruit drop in susceptible genotypes. It is a strong concern for triploid breeding programs aiming to produce seedless mandarin cultivars. The monolocus dominant inheritance of susceptibility, proposed on the basis of diploid population studies, was corroborated in triploid progeny. Bulk segregant analysis coupled with genome scan using a large set of genetically mapped SNP markers and targeted genetic mapping by half tetrad analysis, using SSR and SNP markers, allowed locating a 3.3 Mb genomic region linked to ABS resistance near the centromere of chromosome III. Clusters of resistance genes were identified by gene ontology analysis of this genomic region. Some of these genes are good candidates to control the dominant susceptibility to the ACTtoxin. SSR and SNP markers were developed for efficient early marker-assisted selection of ABS resistant hybrids.

Mots-clés Agrovoc : Citrus, Citrus paradisi, mandarine, Alternaria alternata, résistance aux maladies, résistance génétique, carte génétique, marqueur génétique, triploïdie, Citrus clementina

Mots-clés complémentaires : Citrus deliciosa

Classification Agris : H20 - Maladies des plantes
F30 - Génétique et amélioration des plantes

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations