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Trypanosoma vivax GM6# Antigen: A candidate antigen for diagnosis of african animal trypanosomosis in cattle

Pillay Davita, Izotte Julien, Fikru Regassa, Büscher Philippe, Mucache Hermogenes, Neves Luis, Boulangé Alain, Seck Momar Talla, Bouyer Jérémy, Napier Grant B., Chevtzoff Cyrille, Coustou Virginie, Baltz Théo. 2013. Trypanosoma vivax GM6# Antigen: A candidate antigen for diagnosis of african animal trypanosomosis in cattle. PloS One, 8 (10):e78565, 10 p.

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Quartile : Outlier, Sujet : MULTIDISCIPLINARY SCIENCES

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie; Staps

Abstract : Background Diagnosis of African animal trypanosomosis is vital to controlling this severe disease which hampers development across 10 million km2 of Africa endemic to tsetse flies. Diagnosis at the point of treatment is currently dependent on parasite detection which is unreliable, and on clinical signs, which are common to several other prevalent bovine diseases. Methodology/Principle Findings the repeat sequence of the GM6 antigen of Trypanosoma vivax (TvGM6), a flagellar-associated protein, was analysed from several isolates of T. vivax and found to be almost identical despite the fact that T. vivax is known to have high genetic variation. The TvGM6 repeat was recombinantly expressed in E. coli and purified. An indirect ELISA for bovine sera based on this antigen was developed. The TvGM6 indirect ELISA had a sensitivity of 91.4% (95% CI: 91.3 to 91.6) in the period following 10 days post experimental infection with T. vivax, which decreased ten-fold to 9.1% (95% CI: 7.3 to 10.9) one month post treatment. With field sera from cattle infected with T. vivax from two locations in East and West Africa, 91.5% (95% CI: 83.2 to 99.5) sensitivity and 91.3% (95% CI: 78.9 to 93.1) specificity was obtained for the TvGM6 ELISA using the whole trypanosome lysate ELISA as a reference. For heterologous T. congolense field infections, the TvGM6 ELISA had a sensitivity of 85.1% (95% CI: 76.8 to 94.4). Conclusion/Significance this study is the first to analyse the GM6 antigen of T. vivax and the first to test the GM6 antigen on a large collection of sera from experimentally and naturally infected cattle. This study demonstrates that the TvGM6 is an excellent candidate antigen for the development of a point-of-treatment test for diagnosis of T. vivax, and to a lesser extent T. congolense, African animal trypanosomosis in cattle. (Résumé d'auteur)

Mots-clés Agrovoc : Trypanosoma vivax, Trypanosoma congolense, Épidémiologie, Surveillance épidémiologique, Bétail, Antigène, Test biologique, Diagnostic, Variation génétique

Mots-clés géographiques Agrovoc : Sénégal, Mozambique, Éthiopie

Classification Agris : L73 - Animal diseases
L72 - Pests of animals

Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes

Auteurs et affiliations

  • Pillay Davita, University of KwaZulu-Natal (ZAF)
  • Izotte Julien, CNRS (FRA)
  • Fikru Regassa, Addis Ababa University (ETH)
  • Büscher Philippe, Institute of tropical medecine (BEL)
  • Mucache Hermogenes, Eduardo Mondlane University (MOZ)
  • Neves Luis, Eduardo Mondlane University (MOZ)
  • Boulangé Alain, CIRAD-BIOS-UMR INTERTRYP (MOZ)
  • Seck Momar Talla, ISRA (SEN)
  • Bouyer Jérémy, CIRAD-BIOS-UMR CMAEE (SEN) ORCID: 0000-0002-1913-416X
  • Napier Grant B., Global Alliance for Veterinary Medicines (GBR)
  • Chevtzoff Cyrille, CEVA Santé animale (FRA)
  • Coustou Virginie, CNRS (FRA)
  • Baltz Théo, CNRS (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/571430/)

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