Genome mining reveals a novel and promising NRPS gene cluster in Xanthomonas albilineans, Xanthomonas oryzae and Xanthomonas translucens

Royer Monique, Koebnik Ralf, Marguerettaz Mélanie, Barbe Valérie, Robin Guillaume P., Brin Christelle, Carrère Sébastien, Gomez Camila, Hügelland Manuela, Völler Ginka H., Noëll Julie, Pieretti Isabelle, Rausch Saskia, Verdier Valérie, Poussier Stéphane, Rott Philippe, Süssmuth Roderich, Cociancich Stéphane. 2013. Genome mining reveals a novel and promising NRPS gene cluster in Xanthomonas albilineans, Xanthomonas oryzae and Xanthomonas translucens. In : Resumos do 59° Congresso Brasileiro de Genética,16 a 19 de Setembro de 2013, Águas de Lindóia, Brasil. SBG. s.l. : s.n., Résumé, 30. ISBN 978-85-89109-06-2 Congresso Brasileiro de Genética. 59, Aguas de Lindoia, Brésil, 16 Septembre 2013/19 Septembre 2013.

Note générale : Monique Royer est l'invitée expert du Cirad

Résumé : Various bacteria use non-ribosomal peptide synthesis (NRPS) to produce peptides or other small molecules. These molecules exhibit broad structural diversity and display biological activities that range from adaptation to unfavorable environments, communication or competition with other microorganisms in their natural habitat, or even to action as virulence factors. Conserved features within the NRPS machinery allow the type, and sometimes even the structure, of the synthesized polypeptide to be predicted. Thus, bacterial genome mining via in siIico analyses of NRPS genes offers an attractive opportunity to uncover new bioactive non-ribosomally synthesized peptides. To date, the only known small molecule synthesized by NRPS in the genus Xanthomonas is albicidin produced by Xanthomonas albilineans, a xylem-invading pathogen that causes leaf scald-a lethal disease of sugarcane. In silica analysis of available genomic sequences of Xanthomonas strains led to the discovery of a novel NRPS gene cluster called META-B which doesn't resemble to any gene cluster de- scribed to date. This NRPS gene cluster occurs in (i) X. albilineans, (ii) two pathovars of Xanthomonas oryzae which are the causal agents of two agronomically important diseases of rice (bacterial leaf blight caused by X. oryzae pv. oryzae and bacterial leaf streak caused by X. oryzae pv. oryzicola), and (iii) Xanthomonas translucens , the causal agent of the bacterial leaf streak of wheat. Interestingly, the NRPS gene cluster META-B seems to be specific to strains of Xanthomonas associated with monocotyledonous plants, suggesting a putative involvement in plant-bacteria interactions.

Classification Agris : H20 - Maladies des plantes
F30 - Génétique et amélioration des plantes

Auteurs et affiliations

• Royer Monique, CIRAD-BIOS-UMR BGPI (FRA)
• Koebnik Ralf, IRD (FRA)
• Marguerettaz Mélanie
• Barbe Valérie, Centre national de séquençage (FRA)
• Robin Guillaume P., IRD (FRA)
• Brin Christelle, INRA (FRA)
• Carrère Sébastien, INRA (FRA)
• Gomez Camila, CIRAD-BIOS-UMR BGPI (FRA)
• Hügelland Manuela, Technische Universitaet Berlin (DEU)
• Völler Ginka H., Technische Universitaet Berlin (DEU)
• Noëll Julie, CIRAD-BIOS-UMR BGPI (FRA)
• Pieretti Isabelle, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0009-0003-3498-1230
• Rausch Saskia, Technische Universitaet Berlin (DEU)
• Verdier Valérie, IRD (FRA)
• Poussier Stéphane, Université de la Réunion (REU)
• Rott Philippe, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0001-6085-6159
• Süssmuth Roderich, Technische Universitaet Berlin (DEU)
• Cociancich Stéphane, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0001-8601-3598

Source : Cirad - Agritrop (https://agritrop.cirad.fr/572515/)

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