The emergence of Xanthomonas citri pv. citri in Mali results from at least two independent introductions

Leduc Alice, Vernière Christian, Ravigné Virginie, Magne Maxime, Grygiel Pierre, Juhasz C.C., Boyer Claudine, Vital Karine, Wonni Issa, Traoré Y., Pruvost Olivier. 2013. The emergence of Xanthomonas citri pv. citri in Mali results from at least two independent introductions. In : 11th International Epidemiology Workshop, Beijing, China, 22-25 August 2013. s.l. : s.n., Résumé, 1 p. International Epidemiology Workshop. 11, Pékin, Chine, 22 Août 2013/25 Août 2013.

Résumé : Citrus is threatened by various diseases included Asiatic Citrus Canker, caused by the bacterium Xanthomonas citri pv. Citri (Xcc). This pathogen is listed as a quarantine organism in Europe and is re-emerging in many countries in Africa, including Mali in 2008. Pathogenicity tests showed that Malian strains were related to pathotype A which is pathogenic on a wide range of Citrus species. A Multilocus Sequence Analysis (MLSA) based on six housekeeping genes indicated the presence in Mali of two Sequence Types (ST): ST2 in 4 provinces and ST3, only present in Bamako. A strain collection (n=714) was genotyped with two molecular typing systems using Variable Number of Tandem Repeats (VNTRs). The first system, called MLVA-31, has 31 minisatellite markers and aims to analysis the genetic diversity of a worldwide collection of Xcc. The MLVA-14 called second system has 14 microsatellite markers and is useful for outbreak investigation and epidemics understanding. Both systems data suggested a hypothetical epidemiological link between Malian population and a collection of 46 strains recently sampled in Burkina Faso. Furthermore, analysis of the Malian collection using MLVA-1 revealed a relatively low global genetic diversity (Ht = 0.37). In addition, presence of major clonal complexes supports the hypothesis of a recent emergence. Characterization and structuration of genotyping data defined two independent introductions of the pathogen in Mali. Our results provide additional information on the epidemiology of Xcc and its re-emergence in Africa and point out the importance of MLVA-based genotyping tools in molecular epidemiology analyses and global surveillance.

Classification Agris : H20 - Maladies des plantes

Auteurs et affiliations

• Leduc Alice, CIRAD-BIOS-UMR PVBMT (REU)
• Vernière Christian, CIRAD-BIOS-UMR PVBMT (REU) ORCID: 0000-0002-2312-2073
• Ravigné Virginie, CIRAD-BIOS-UMR BGPI (REU) ORCID: 0000-0002-4252-2574
• Magne Maxime
• Grygiel Pierre, CIRAD-BIOS-UMR PVBMT (REU)
• Juhasz C.C., Université de la Réunion (REU)
• Boyer Claudine, CIRAD-BIOS-UMR PVBMT (REU) ORCID: 0009-0009-3084-5593
• Vital Karine, CIRAD-BIOS-UMR PVBMT (REU)
• Wonni Issa, INERA (BFA)
• Traoré Y.
• Pruvost Olivier, CIRAD-BIOS-UMR PVBMT (REU)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/572715/)

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