DNA and RNA polymerase activity in a Moniliophthora perniciosa mitochondrial plasmid and self-defense against oxidative stress

Andrade Bruno Silva, Villela Dias Cristianao, Gomes Dayane Santos, Micheli Fabienne, Goes-Neto Aristoteles. 2013. DNA and RNA polymerase activity in a Moniliophthora perniciosa mitochondrial plasmid and self-defense against oxidative stress. Genetics and Molecular Research, 12 (2) : 1944-1950.

Quartile : Q4, Sujet : GENETICS & HEREDITY / Quartile : Q4, Sujet : BIOCHEMISTRY & MOLECULAR BIOLOGY

Résumé : Moniliophthora perniciosa (Stahel) Aime and Phillips-Mora is a hemibiotrophic basidiomycete (Agaricales, Tricholomataceae) that causes witches' broom disease in cocoa (Theobroma cacao L.). This pathogen carries a stable integrated invertron-type linear plasmid in its mitochondrial genome that encodes viral-like DNA and RNA polymerases related to fungal senescence and longevity. After culturing the fungus and obtaining its various stages of development in triplicate, we carried out total RNA extraction and subsequent complementary DNA synthesis. To analyze DNA and RNA polymerase expression levels, we performed real-time reverse transcriptase polymerase chain reaction for various fungal phases of development. Our results showed that DNA and RNA polymerase gene expression in the primordium phase of M. perniciosa is related to a potential defense mechanism against T. cacao oxidative attack.

Mots-clés Agrovoc : Theobroma cacao, Moniliophthora, mécanisme de défense, oxydation, stress, plasmide, mitochondrie, Transcription inverse, activité enzymatique, expression des gènes

Mots-clés géographiques Agrovoc : Brésil

Mots-clés complémentaires : Moniliophthora perniciosa

Classification Agris : H20 - Maladies des plantes
F30 - Génétique et amélioration des plantes

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Andrade Bruno Silva, UESB (BRA)
• Villela Dias Cristianao, UESC (BRA)
• Gomes Dayane Santos, UESC (BRA)
• Micheli Fabienne, CIRAD-BIOS-UMR AGAP (BRA)
• Goes-Neto Aristoteles, UEFS (BRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/573036/)

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