An international collaborative study to determine the prevalence of contagious caprine pleuropneumonia by monoclonal antibody-based cELISA

Peyraud Armelle, Poumarat François, Tardy Florence, Manso-Silvan Lucia, Hamroev Karomatullo, Tilloev Tillo, Amirbekov Mulojon, Tounkara Karim, Bodjo Sanne Charles, Wesonga Hezron, Gacheri Nkando Isabel, Jenberie Shiferaw, Yami Martha, Cardinale Eric, Meenowa Deodass, Jaumally Mahmad Reshad, Yaqub Tahir, Zubair Shabbir Muhammad, Mukhtar Nadia, Halimi Mohibullah, Ziay Ghulan Mohammad, Schauwers Willy, Noori Hafizullah, Rajabi Ali Madad, Ostrowski Stéphane, Thiaucourt François. 2014. An international collaborative study to determine the prevalence of contagious caprine pleuropneumonia by monoclonal antibody-based cELISA. BMC Veterinary Research, 10 (48), 9 p.

Quartile : Q1, Sujet : VETERINARY SCIENCES

Résumé : Background: Few serological tests are available for detecting antibodies against Mycoplasma capricolum subsp. capripneumoniae, the causal agent of contagious caprine pleuropneumonia (CCPP). The complement fixation test, the test prescribed for international trade purposes, uses a crude antigen that cross-reacts with all the other mycoplasma species of the "mycoides cluster" frequently infecting goat herds. The lack of a more specific test has been a real obstacle to the evaluation of the prevalence and economic impact of CCPP worldwide. A new competitive ELISA kit for CCPP, based on a previous blocking ELISA, was formatted at CIRAD and used to evaluate the prevalence of CCPP in some regions of Kenya, Ethiopia, Mauritius, Tajikistan and Pakistan in an international collaborative study. Results: The strict specificity of the test was confirmed in CCPP-free goat herds exposed to other mycoplasma species of the ''mycoides cluster". Prevalence studies were performed across the enzootic range of the disease in Africa and Asia. Seroprevalence was estimated at 14.6% in the Afar region of Ethiopia, whereas all the herds presented for CCPP vaccination in Kenya tested positive (individual seroprevalence varied from 6 to 90% within each herd). In Mauritius, where CCPP emerged in 2009, nine of 62 herds tested positive. In Central Asia, where the disease was confirmed only recently, no positive animals were detected in the Wakhan District of Afghanistan or across the border in neighboring areas of Tajikistan, whereas seroprevalence varied between 2.7% and 44.2% in the other districts investigated and in northern Pakistan. The test was also used to monitor seroconversion in vaccinated animals. Conclusions: This newly formatted CCPP cELISA kit has retained the high specificity of the original kit. It can therefore be used to evaluate the prevalence of CCPP in countries or regions without vaccination programs. It could also be used to monitor the efficacy of vaccination campaigns as high-quality vaccines induce high rates of seroconversion.

Mots-clés géographiques Agrovoc : Éthiopie, Maurice, Pakistan, Tadjikistan, Kenya

Classification Agris : L73 - Maladies des animaux

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

• Peyraud Armelle, CIRAD-BIOS-UMR CMAEE (FRA) ORCID: 0009-0002-1616-7588
• Poumarat François, ANSES (FRA)
• Tardy Florence, ANSES (FRA)
• Manso-Silvan Lucia, CIRAD-BIOS-UMR CMAEE (FRA) ORCID: 0000-0002-5539-3804
• Hamroev Karomatullo, State Veterinary Inspection Services (TJK)
• Tilloev Tillo, State Veterinary Inspection Services (TJK)
• Amirbekov Mulojon, State Veterinary Inspection Services (TJK)
• Tounkara Karim, Pan African Veterinary Vaccine Centre (ETH)
• Bodjo Sanne Charles, Pan African Veterinary Vaccine Centre (ETH)
• Wesonga Hezron, KARI (KEN)
• Gacheri Nkando Isabel, KARI (KEN)
• Jenberie Shiferaw, NVI (ETH)
• Yami Martha, NVI (ETH)
• Cardinale Eric, CIRAD-BIOS-UMR CMAEE (REU) ORCID: 0000-0002-3434-3541
• Meenowa Deodass, Ministère de l'agriculture, de la technologie alimentaire et des ressources naturelles (Maurice) (MUS)
• Jaumally Mahmad Reshad, Ministère de l'agriculture, de la technologie alimentaire et des ressources naturelles (Maurice) (MUS)
• Yaqub Tahir, University of Veterinary and Animal Sciences (PAK)
• Zubair Shabbir Muhammad, University of Veterinary and Animal Sciences (PAK)
• Mukhtar Nadia, University of Veterinary and Animal Sciences (PAK)
• Halimi Mohibullah, Ministry of Agriculture, Irrigation and Livestock (Afghanistan) (AFG)
• Ziay Ghulan Mohammad, Ministry of Agriculture, Irrigation and Livestock (Afghanistan) (AFG)
• Schauwers Willy, Ministry of Agriculture, Irrigation and Livestock (Afghanistan) (AFG)
• Noori Hafizullah, Wildlife Conservation Society (AFG)
• Rajabi Ali Madad, Wildlife Conservation Society (AFG)
• Ostrowski Stéphane, Wildlife Conservation Society (USA)
• Thiaucourt François, CIRAD-BIOS-UMR CMAEE (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/575212/)

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