What do Pneumocystis organisms tell us about the phylogeography of their hosts? The case of the woodmouse Apodemus sylvaticus in Continental Europe and Western Mediterranean Islands

Demanche Christine, Deville Manjula, Michaux Johan, Barriel Véronique, Pinçon Claire, Aliouat-Denis Cécile, Pottier Muriel, Noël Christophe, Viscogliosi Eric, Aliouat El Moukhtar, Dei-Cas Eduardo, Morand Serge, Guillot Jacques. 2015. What do Pneumocystis organisms tell us about the phylogeography of their hosts? The case of the woodmouse Apodemus sylvaticus in Continental Europe and Western Mediterranean Islands. PloS One, 10 (3):e0120839, 17 p.

Quartile : Q1, Sujet : MULTIDISCIPLINARY SCIENCES

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie; Staps

Résumé : Pneumocystis fungi represent a highly diversified biological group with numerous species, which display a strong host-specificity suggesting a long co-speciation process. In the present study, the presence and genetic diversity of Pneumocystis organisms was investigated in 203 lung samples from woodmice (Apodemus sylvaticus) collected on western continental Europe and Mediterranean islands. The presence of Pneumocystis DNA was assessed by nested PCR at both large and small mitochondrial subunit (mtLSU and mtSSU) rRNA loci. Direct sequencing of nested PCR products demonstrated a very high variability among woodmouse-derived Pneumocystis organisms with a total number of 30 distinct combined mtLSU and mtSSU sequence types. However, the genetic divergence among these sequence types was very low (up to 3.87%) and the presence of several Pneumocystis species within Apodemus sylvaticus was considered unlikely. The analysis of the genetic structure of woodmouse-derived Pneumocystis revealed two distinct groups. The first one comprised Pneumocystis from woodmice collected in continental Spain, France and Balearic islands. The second one included Pneumocystis from woodmice collected in continental Italy, Corsica and Sicily. These two genetic groups were in accordance with the two lineages currently described within the host species Apodemus sylvaticus. Pneumocystis organisms are emerging as powerful tools for phylogeographic studies in mammals.

Classification Agris : L72 - Organismes nuisibles des animaux
L73 - Maladies des animaux
S50 - Santé humaine

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

• Demanche Christine, Université de Lille (FRA)
• Deville Manjula, ENVA (FRA)
• Michaux Johan, Université de Liège (BEL)
• Barriel Véronique, Université de Liège (BEL)
• Pinçon Claire, Université de Lille (FRA)
• Aliouat-Denis Cécile, Université de Lille (FRA)
• Pottier Muriel, Université de Lille (FRA)
• Noël Christophe, INEX Business Centre (GBR)
• Viscogliosi Eric, Institut Pasteur (FRA)
• Aliouat El Moukhtar, Université de Lille (FRA)
• Dei-Cas Eduardo, Institut Pasteur (FRA)
• Morand Serge, CIRAD-ES-UPR AGIRs (LAO) ORCID: 0000-0003-3986-7659
• Guillot Jacques, ENVA (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/575925/)

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