Functional analysis of CcDREB1D promoter region from haplotypes of Coffea canephora through genetic transformation of Nicotiana tabacum

De Aquino Sinara Oliveira, Carneiro Fernanda A, Rego Erica C.S., Alves Gabriel Sergio Costa, Marraccini Pierre, Andrade Alan Carvalho. 2015. Functional analysis of CcDREB1D promoter region from haplotypes of Coffea canephora through genetic transformation of Nicotiana tabacum. In : Proceedings of the 25th International Conference on Coffee Science. ASIC. Paris : ASIC, 42-46. ISBN 978-2-900212-24-0 International Conference on Coffee Science. 25, Armenia, Colombie, 8 Septembre 2014/13 Septembre 2014.

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Résumé : In many higher plants, DREB genes were shown to be involved in the transduction pathways of abiotic stress. Previous results showed that CcDREB1Dgene expression increased under drought stress in leaves of drought-tolerant clone 14 of Coffea canephora Conilon but not in those of the drought-susceptible clone 22. By sequencing the DREB1Dpromoter regions of these two clones, several nucleic polymorphisms (“single nucleotide polymorphism” [SNP] and insertion/deletion [INDELs]) were found. In order to know if these polymorphisms could explain the differences of DREB1Dgene expression observed between the clones 14 and 22, 5' deletions of several alleles of the CcDREB1Dpromoter regions were made and cloned independently into the binary vector pBI101 in order to analyze their ability to control the expression of the uidA reporter gene in transgenic tobacco (Nicotiana tabacum) plants. This work shows preliminary results of DREB1Dpromoter constructs analyzed in leaves of N. tabacum.

Classification Agris : F30 - Génétique et amélioration des plantes
H50 - Troubles divers des plantes
F61 - Physiologie végétale - Nutrition

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