Value of routine dengue Diagnostic tests in urine and saliva specimens

Andries Anne-Claire, Duong Veasna, Ly Sowath, Cappelle Julien, Srorn Kim Kim, Lorn Try Patrich, Ros Sopheaktra, Ong Sivuth, Huy Rekol, Horwood Paul, Flamand Marie, Sakuntabhai Anavaj, Tarantola Arnaud, Buchy Philippe. 2015. Value of routine dengue Diagnostic tests in urine and saliva specimens. PLoS Neglected Tropical Diseases, 9 (9):e0004100, 30 p.

Journal article ; Article de recherche ; Article de revue à facteur d'impact Revue en libre accès total
Published version - Anglais
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Quartile : Q1, Sujet : TROPICAL MEDICINE / Quartile : Q1, Sujet : PARASITOLOGY

Abstract : Dengue is the most important arthropod-borne disease affecting humans and represents a huge public health burden in affected countries. Symptoms are often non-specific hence the need for an early, sensitive and specific diagnosis of dengue for appropriate management as well as for early epidemic detection. Currently, almost all laboratory diagnostic methods require a blood specimen that may be sometimes be difficult or inconvenient to obtain. In this study, we assessed the possibility to use saliva and urine samples as alternatives to blood specimens in dengue diagnosis. We demonstrated that the performances of the different diagnostic methods (RT-PCR, NS1 antigen detection and anti-DENV IgM/IgG/IgA ELISAs) were in general not as good in saliva and urine as in plasma, but that the use of these body fluids obtained by non-invasive methods could be of value in certain circumstances such as outbreak investigations or in young children (once they are old enough to comply to instructions), in addition to the situations when blood cannot be easily collected (e.g., lack of phlebotomist, refusal of the procedure, etc.). (Résumé d'auteur)

Mots-clés Agrovoc : Flavivirus, Test biologique, Diagnostic de laboratoire, Salive, Sang, Urine, Santé publique, Genre humain, Technique analytique

Mots-clés géographiques Agrovoc : Cambodge

Mots-clés complémentaires : Virus de la dengue

Classification Agris : L73 - Animal diseases
000 - Other themes
U30 - Research methods

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

  • Andries Anne-Claire, Institut Pasteur du Cambodge (KHM)
  • Duong Veasna, Institut Pasteur du Cambodge (KHM)
  • Ly Sowath, Institut Pasteur du Cambodge (KHM)
  • Cappelle Julien, CIRAD-ES-UPR AGIRs (KHM) ORCID: 0000-0001-7668-1971
  • Srorn Kim Kim, Kampong Cham Provincial Hospital (KHM)
  • Lorn Try Patrich, Kampong Cham Provincial Hospital (KHM)
  • Ros Sopheaktra, Institut Pasteur du Cambodge (KHM)
  • Ong Sivuth, Institut Pasteur du Cambodge (KHM)
  • Huy Rekol, Ministère de la santé (Cambodge) (KHM)
  • Horwood Paul, Institut Pasteur du Cambodge (KHM)
  • Flamand Marie, Institut Pasteur (FRA)
  • Sakuntabhai Anavaj, Institut Pasteur (FRA)
  • Tarantola Arnaud, Institut Pasteur du Cambodge (KHM)
  • Buchy Philippe, Institut Pasteur du Cambodge (KHM)

Source : Cirad-Agritrop (

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