Occurrence of Awka wilt disease of coconut in Nigeria for one century

Osagie Isaac Jesuorobo, Ojomo Edward Eghomwan, Pilet Fabian. 2015. Occurrence of Awka wilt disease of coconut in Nigeria for one century. Phytopathogenic Mollicutes, 5, suppl. 1 : S61-S62. Annual Meeting of the International Phytoplasmologist Working Group. 3, Balaclava, Maurice, 14 Janvier 2015/17 Janvier 2015.

Url - éditeur : http://mauritius2015.ipwgnet.org/

Résumé : This study was conducted to characterize phytoplasmas from coconut palms showing symptoms of Awka wilt disease in south and south-east Nigeria and determine if there is geographic differentiation among the phytoplasma strains. Stem samples were collected from symptomatic coconut palms in three region of Nigeria. For each sample, DNA was extracted and the 16S rDNA was amplified by PCR by using the P1/P7 primers. Amplicons were sequenced for five Nigerian strains. The sequences were compared with each other, with LDN (lethal decline Nigeria) sequence published 20 years ago, and with sequences from Ghana. A homology of 100% was observed among the 16S rDNA sequence published and the sequences of the five strains under investigation. Based on the known conservation of 16S rDNA sequences, this result suggests a relative stability of the lethal yellowing disease phytoplasma strains in the southern regions of Nigeria, and no introduction of the “Ghanaian strain”.

Mots-clés Agrovoc : Cocos nucifera, maladie bactérienne, phytoplasme, identification, PCR, provenance

Mots-clés géographiques Agrovoc : Nigéria

Mots-clés complémentaires : Jaunissement mortel

Classification Agris : H20 - Maladies des plantes

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

• Osagie Isaac Jesuorobo, NIFOR (NGA)
• Ojomo Edward Eghomwan, NIFOR (NGA)
• Pilet Fabian, CIRAD-BIOS-UMR PVBMT (REU) ORCID: 0000-0003-0079-216X

Source : Cirad-Agritrop (https://agritrop.cirad.fr/578008/)

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