Establishment of in vitro multiple bud cultures and plant regeneration in Phoenix dactylifera L. cv Barhee

Fki Lotfi, Bouaziz Neila, Kriaa Walid, Masmoudi R., Gargouri-Bouzid Radhia, Rival Alain, Drira Noureddine. 2010. Establishment of in vitro multiple bud cultures and plant regeneration in Phoenix dactylifera L. cv Barhee. In : Palms 2010, Biology of the palm family (Abstracts books) : International Symposium 5-7 May 2010, Montpellier, France. Rival Alain (ed.). s.l. : s.n., Résumé, 78. International Symposium on the Biology of the Palm Family, Montpellier, France, 5 Mai 2010/7 Mai 2010.

Résumé : Adventitious bud clusters of date palm cv Barhee were successfully established from juvenile leaves sampled from offshoots. An average 8.4 + 1.3 adventitious buds per juvenile leaf (< 1cm) was obtained using MS basal medium supplemented with reduced amounts of 2,4-D (0.2 mg/L). Explants bearing buds were transferred onto MS medium supplemented with 70 g/L sucrose in order to promote the proliferation of caulogenic culture able to generate multiple bud clusters. The promoting effect of temporary immersion on shoots proliferation was found to be significant when compared to cultivation on solid media. Indeed, multiplication rate increased from 2 to 3. Elongation of shoots was achieved using a thin-film of liquid medium deprived of PGRs. Further rooting was induced in liquid MS basal medium supplemented with 4 mg/L IBA. Twelve-month old vitroplants were physiologically able to withstand acclimatization. As a result, 75% of regenerated plants were successfully acclimatized. No phenotypic variation was observed among more than 500 adventitious bud-derived plants. All regenerants have survived after field transplantation.

Classification Agris : F02 - Multiplication végétative des plantes
F62 - Physiologie végétale - Croissance et développement

Auteurs et affiliations

• Fki Lotfi, Faculté des sciences de Sfax (TUN)
• Bouaziz Neila, Faculté des sciences de Sfax (TUN)
• Kriaa Walid, Faculté des sciences de Sfax (TUN)
• Masmoudi R., Faculté des sciences de Sfax (TUN)
• Gargouri-Bouzid Radhia, Faculté des sciences de Sfax (TUN)
• Rival Alain, CIRAD-BIOS-UMR DIADE (FRA) ORCID: 0000-0001-6304-3268
• Drira Noureddine, Faculté des sciences de Sfax (TUN)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/578122/)

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