Current challenges for de novo Hevea brasiliensis genome sequencing

Montoro Pascal, Tang Chaorong, Hu Songnian, Jayasekaran Kandakumar, Kolesnikova-Allen Maria. 2015. Current challenges for de novo Hevea brasiliensis genome sequencing. In : Proceedings International Rubber Conference 2015: Productivity and quality towards a sustainable and profitable natural rubber sector. Le Quang Khoi (ed.). IRRDB, RRIV. Ho Chi Minh City : Agricultural Publishing House, 80-84. ISBN 978-604-60-2156-8 International Rubber Conference 2015, Ho Chi Minh City, Viet Nam, 2 Novembre 2015/3 Novembre 2015.

Résumé : The fRRDB Biotechnology Group recommended in 2009 the generation of a reference sequence of rubber genome in order to ease resequencing of genome for commercial clones, to accelerate breeding programmes, and to improve functional understanding of plants and consequent rubber production. So far, several national initiatives have been launched in China, Malaysia and United Kingdom, and likely in Japan and Thailand. Although genome sequence of major crops is available, only a draft sequence was published for rubber. This paper presents an overview of these different initiatives. De novo sequencing was carried out on clones Reyan7-33-97, RRlM 600, and RRfM 928. These genomes were assembled in a large number of scaffolds (7,700; 608,017 and 954,373 scaffolds, respectively) far from the 18 chromosomes of the haploid rubber genome. Rubber clones are highly heterozygous and have a high frequency of repeat sequences. These constraints are supposed to hamper the delivery of a full sequence of rubber genome. New strategies for de novo sequencing and interactions between these different initiatives were suggested to overcome these issues.

Classification Agris : F30 - Génétique et amélioration des plantes

Auteurs et affiliations

• Montoro Pascal, CIRAD-BIOS-UMR AGAP (FRA)
• Tang Chaorong, Rubber Research Institute (CHN)
• Hu Songnian, CAS (CHN)
• Jayasekaran Kandakumar, National University of Singapore (SGP)
• Kolesnikova-Allen Maria, TARRC (GBR)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/579084/)

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