Comparison of the affinity and extraction yield of trace amounts of three Cry proteins from Bacillus thuringiensis in contrasting types of soil

Hung Truong Phuc, Truong Le Van, Binh Ngo Dinh, Frutos Roger, Quiquampoix Hervé, Staunton Siobhán. 2016. Comparison of the affinity and extraction yield of trace amounts of three Cry proteins from Bacillus thuringiensis in contrasting types of soil. European Journal of Soil Science, 67 (1), n.spéc. issue on Including Landmark Papers 5 : 90-98.

Quartile : Q1, Sujet : SOIL SCIENCE

Résumé : The use of insecticidal proteins known as Cry or Bt, either as biopesticides used in agriculture or as vector control or originating from commercial genetically modified crops (GM), is increasing rapidly. The fate of these proteins in the environment depends strongly on their adsorption on the organo–mineral complexes of soil. Environmental monitoring requires the quantification of the proteins and this entails their chemical extraction from soil. Three Cry proteins, Cry1Ac, Cry1C and Cry2A, present in commercial biopesticide formulations or synthesized by GM plants or both were studied. The adsorption of trace amounts of Cry proteins on over 40 types of soil with contrasting properties was measured in dilute suspension. After a short incubation the extraction yield was measured with a previously tested alkaline solution that contained surfactant and another protein. Each of the proteins had a strong affinity for soil. No soil property was observed to determine either the affinity for soil or the extraction yield. There was no simple relation between the affinity (assessed from the distribution coefficient, Kd) and the extraction yield, although there was a significant inverse relation (P < 0.05) for two of the proteins, Cry1Ac and Cry2A. The proteins differ in both their affinity for soil and their extraction yields. We conclude that these insecticidal proteins will be largely immobile in soil, but that routine environmental monitoring can give only semi-quantitative values for protein in soil.

Mots-clés Agrovoc : Bacillus thuringiensis, biopesticide, protéine bactérienne, type de sol, persistance des pesticides, pollution du sol, pollution par l'agriculture, adsorption

Mots-clés géographiques Agrovoc : France

Classification Agris : H02 - Pesticides
P02 - Pollution
P33 - Chimie et physique du sol

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

• Hung Truong Phuc, INRA (FRA)
• Truong Le Van, INRA (FRA)
• Binh Ngo Dinh, Institute of Biotechnology (VNM)
• Frutos Roger, CIRAD-BIOS-UMR INTERTRYP (FRA)
• Quiquampoix Hervé, INRA (FRA)
• Staunton Siobhán, INRA (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/580033/)

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