Hung Truong Phuc, Truong Le Van, Binh Ngo Dinh, Frutos Roger, Quiquampoix Hervé, Staunton Siobhán. 2016. Persistence of detectable insecticidal proteins from Bacillus thuringiensis (Cry) and toxicity after adsorption on contrasting soils. Environmental Pollution, 208 (Part B) : 318-325.
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Quartile : Q1, Sujet : ENVIRONMENTAL SCIENCES
Liste HCERES des revues (en SHS) : oui
Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie
Résumé : Insecticidal Cry, or Bt, proteins are produced by the soil-endemic bacterium, Bacillus thuringiensis and some genetically modified crops. Their environmental fate depends on interactions with soil. Little is known about the toxicity of adsorbed proteins and the change in toxicity over time. We incubated Cry1Ac and Cry2A in contrasting soils subjected to different treatments to inhibit microbial activity. The toxin was chemically extracted and immunoassayed. Manduca sexta was the target insect for biotests. Extractable toxin decreased during incubation for up to four weeks. Toxicity of Cry1Ac was maintained in the adsorbed state, but lost after 2 weeks incubation at 25 °C. The decline in extractable protein and toxicity were much slower at 4 °C with no significant effect of soil sterilization. The major driving force for decline may be time-dependent fixation of adsorbed protein, leading to a decrease in the extraction yield in vitro, paralleled by decreasing solubilisation in the larval gut.
Mots-clés Agrovoc : Bacillus thuringiensis, biopesticide, persistance des pesticides, sol, adsorption, toxicité du sol, pollution du sol, pollution par l'agriculture, protéine bactérienne
Mots-clés géographiques Agrovoc : Languedoc-Roussillon, France
Classification Agris : H02 - Pesticides
P02 - Pollution
P33 - Chimie et physique du sol
Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes
Auteurs et affiliations
- Hung Truong Phuc, INRA (FRA)
- Truong Le Van, INRA (FRA)
- Binh Ngo Dinh, Institute of Biotechnology (VNM)
- Frutos Roger, CIRAD-BIOS-UMR INTERTRYP (FRA)
- Quiquampoix Hervé, INRA (FRA)
- Staunton Siobhán, INRA (FRA)
Source : Cirad-Agritrop (https://agritrop.cirad.fr/580066/)
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