Immunomodulating properties of Amblyomma variegatum saliva and role on tick-borne disease transmission

Chamayou Léo, Rodrigues Valérie, Fernandez Bernard, Andersen Alexandre, Demettre Edith, Seveno Martial, Vigy Oana, Aprelon Rosalie, Giraud-Girard Ken, Stachurski Frédéric, Vachiéry Nathalie, Holzmuller Philippe. 2016. Immunomodulating properties of Amblyomma variegatum saliva and role on tick-borne disease transmission. In : 10es journées du réseau français "Immunologie des animaux domestiques". Ploufragan : s.n., 1 p. IAD 2016 - 10es journées du réseau français "Immunologie des animaux domestiques", Ploufragan, France, 17 Mars 2016/18 Mars 2016.

Résumé : The tropical bont tick, Amblyomma variegatum, is a major pest of ruminants, causing direct skin lesions, vectoring the obligate intracellular ricketsiale Ehrlichia ruminantium, the causative agent of heartwater, and being able to reactivate dermatophilosis. In general, tick salivary proteins are the result of host blood feeding adaptation and are known to contain inhibitors of blood clotting, platelet aggregation and angiogenesis, as well as vasodilators and immunomodulators. The general objective of this study was to better understand the role of the saliva in the tick interaction with the host but also in pathogen transmission or diseases activation. We therefore first analysed the immunomodulating properties of semi-fed A. variegatum female saliva on bovine peripheral blood mononuclear cells (PBMCs) in vitro. Flow cytometry and cytokine ELISAs have been used to evaluate the saliva impact on PBMCs. We focused on immunosuppressive properties by analysing both lymphocytes proliferation and monocyte-derived macrophages phenotype characterised by inhibition or induction of stimulatory and co-stimulatory molecules such as MHC-II, CD40, CD80 or CD86, and production of pro- or anti-inflammatory cytokines such as interleukin (IL)-2, IL-4, IL-6, IL-10, IL-12p40, and tumor necrosis factor (TNF)-α. Moreover, a proteomics exhaustive molecular characterisation of A. variegatum saliva was performed to cluster data set according to the evidenced immunomodulatory properties, and allowed refined characterisation of Amblyomma sialome. Bioinformatics functional analysis of tick saliva highlighted molecular determinants that could, at least in part, explain the biological effects observed on bovine PBMCs. Our results bring new insights for a better understanding of tick-ruminant interactions, and open new perspectives to develop integrative strategies to interfere with the infectious pathoimmunological process of such tick-borne infections.

Mots-clés libres : Amblyomma variegatum, Tick saliva, Immunomodulation, Proteomics

Classification Agris : L72 - Organismes nuisibles des animaux
L73 - Maladies des animaux

Auteurs et affiliations

• Chamayou Léo
• Rodrigues Valérie, CIRAD-BIOS-UMR CMAEE (FRA)
• Fernandez Bernard, CIRAD-BIOS-UMR CMAEE (FRA)
• Andersen Alexandre
• Demettre Edith, CNRS (FRA)
• Seveno Martial, Institut de génomique fonctionnelle (FRA)
• Vigy Oana, Institut de génomique fonctionnelle (FRA)
• Aprelon Rosalie, CIRAD-BIOS-UMR CMAEE (GLP)
• Giraud-Girard Ken, CIRAD-BIOS-UMR CMAEE (GLP)
• Stachurski Frédéric, CIRAD-BIOS-UMR CMAEE (FRA) ORCID: 0009-0009-5904-3375
• Vachiéry Nathalie, CIRAD-BIOS-UMR CMAEE (FRA)
• Holzmuller Philippe, CIRAD-BIOS-UMR CMAEE (FRA) ORCID: 0000-0002-8919-9081

Source : Cirad-Agritrop (https://agritrop.cirad.fr/580634/)

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