How marker-assisted breeding of Musa balbisiana genitors devoid of infectious endogenous Banana streak virus sequences contributes to pesticide-free agroecological banana farming systems

Umber Marie, Pichaut Jean-Philippe, Farinas Benoît, Laboureau Nathalie, Janzac Bérenger, Pineau Kaïssa, Pressat Gersende, Baurens Franc-Christophe, Chabannes Matthieu, Duroy Pierre-Olivier, Guiougou Chantal, Delos Jean-Marie Eric, Jenny Christophe, Iskra Caruana Marie-Line, Salmon Frédéric, Teycheney Pierre-Yves. 2016. How marker-assisted breeding of Musa balbisiana genitors devoid of infectious endogenous Banana streak virus sequences contributes to pesticide-free agroecological banana farming systems. In : Engineering Ecological Modernization of Agriculture - Exploring the Potential of Tropical Biological Resources for Innovation - Towards a Bio-Economic Development of Caribbean Countries : Proceedings of the 52nd annual meeting of the CFCS. Naves Michel (ed.), Angeon Valérie (ed.), Merlot Bérengère (ed.), Fahrasmane Louis (ed.), Diman Jean Louis (ed.), Labbé Patrick (ed.), Traffond Patricia (ed.), Colon Wilfredo (ed.), Ozier Lafontaine Harry (ed.). Gosier : CFCS, 75-76. Annual Meeting of the Caribbean Food Crops Society. 52, Gosier, Guadeloupe, 10 Juillet 2016/16 Juillet 2016.

Résumé : Breeding new interspecific banana hybrid varieties with pests and disease resistance characters is instrumental for the development of pesticide-free agroecological banana farming systems. Such breeding relies on the combined use of Musa acuminata and M. balbisiana parents. Unfortunately, infectious alleles of endogenous Banana streak virus (eBSV) sequences are present in the genome of M. balbisiana genitors. Upon activation by biotic and abiotic stresses, these infectious eBSVs lead to spontaneous infections by several species of Banana streak virus (BSV) in interspecific hybrids harbouring both M. acuminata and M. balbisiana genomes. In this work, we show that M. balbisiana diploid genitors available for breeding host at least one infectious eBSV. We also show how we segregated infectious and non-infectious eBSV alleles in seedy M. balbisiana diploids through self-pollination or chromosome doubling of haploid lines. We report on the successful breeding of M. balbisiana diploid genitors devoid of all infectious eBSV alleles following self-pollination and on the potential of breeding additional M. balbisiana diploid genitors free of infectious eBSVs by crossing parents displaying complementary eBSV patterns. This work paves the way to the safe use of M. balbisiana genitors for breeding banana interspecific hybrid varieties with no risk of activation of infectious eBSVs.

Mots-clés libres : Musa, Endogenous viral element, Banana streak virus, Infectious alleles, Marker-assisted breeding

Classification Agris : H20 - Maladies des plantes
F30 - Génétique et amélioration des plantes

Auteurs et affiliations

• Umber Marie, INRA (GLP)
• Pichaut Jean-Philippe
• Farinas Benoît
• Laboureau Nathalie, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0002-0105-3919
• Janzac Bérenger
• Pineau Kaïssa, CIRAD-BIOS-UMR AGAP (GLP) ORCID: 0009-0000-1998-4618
• Pressat Gersende, CIRAD-BIOS-UMR AGAP (GLP)
• Baurens Franc-Christophe, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-5219-8771
• Chabannes Matthieu, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0001-5754-5982
• Duroy Pierre-Olivier, CIRAD-BIOS-UMR BGPI (FRA)
• Guiougou Chantal, CIRAD-BIOS-UMR AGAP (GLP)
• Delos Jean-Marie Eric, CIRAD-BIOS-UMR AGAP (GLP)
• Jenny Christophe, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-6165-6888
• Iskra Caruana Marie-Line, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0003-4486-2449
• Salmon Frédéric, CIRAD-BIOS-UMR AGAP (GLP)
• Teycheney Pierre-Yves, CIRAD-BIOS-UMR AGAP (GLP) ORCID: 0000-0002-9754-0745

Source : Cirad-Agritrop (https://agritrop.cirad.fr/580756/)

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