Comparative analysis of a sex chromosome from the blackchin tilapia, Sarotherodon melanotheron

Gammerdinger William J., Conte Matthew A., Baroiller Jean-François, D'Cotta Helena, Kocher Thomas D.. 2016. Comparative analysis of a sex chromosome from the blackchin tilapia, Sarotherodon melanotheron. BMC Genomics, 17 (808), 10 p.

Url - jeu de données - Entrepôt autre : https://trace.ncbi.nlm.nih.gov/Traces/sra/?run=SRR1606298

Quartile : Q1, Sujet : BIOTECHNOLOGY & APPLIED MICROBIOLOGY / Quartile : Q2, Sujet : GENETICS & HEREDITY

Résumé : Background Inversions and other structural polymorphisms often reduce the rate of recombination between sex chromosomes, making it impossible to fine map sex-determination loci using traditional genetic mapping techniques. Here we compare distantly related species of tilapia that each segregate an XY system of sex-determination on linkage group 1. We use whole genome sequencing to identify shared sex-patterned polymorphisms, which are candidates for the ancestral sex-determination mutation. Results We found that Sarotherodon melanotheron segregates an XY system on LG1 in the same region identified in Oreochromis niloticus. Both species have higher densities of sex-patterned SNPs, as well as elevated number of ancestral copy number variants in this region when compared to the rest of the genome, but the pattern of differentiation along LG1 differs between species. The number of sex-patterned SNPs shared by the two species is small, but larger than expected by chance, suggesting that a novel Y-chromosome arose just before the divergence of the two species. We identified a shared sex-patterned SNP that alters a Gata4 binding site near Wilms tumor protein that might be responsible for sex-determination. Conclusions Shared sex-patterned SNPs, insertions and deletions suggest an ancestral sex-determination system that is common to both S. melanotheron and O. niloticus. Functional analyses are needed to evaluate shared SNPs near candidate genes that might play a role in sex-determination of these species. Interspecific variation in the sex chromosomes of tilapia species provides an excellent model system for understanding the evolution of vertebrate sex chromosomes.

Mots-clés Agrovoc : Oreochromis niloticus, polymorphisme génétique, Tilapia

Mots-clés géographiques Agrovoc : Sénégal

Classification Agris : L10 - Génétique et amélioration des animaux
L53 - Physiologie animale - Reproduction
M12 - Production de l'aquaculture

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

Auteurs et affiliations

• Gammerdinger William J., University of Maryland (USA)
• Conte Matthew A., University of Maryland (USA)
• Baroiller Jean-François, CIRAD-PERSYST-UMR ISEM (FRA) ORCID: 0000-0002-0992-1725
• D'Cotta Helena, CIRAD-PERSYST-UMR ISEM (FRA) ORCID: 0000-0003-2739-3476
• Kocher Thomas D., University of Maryland (USA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/582366/)

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