The source of latex. Tracing carbon from leaf photosynthesis to latex metabolism in rubber trees using carbon stable isotopes

Thaler Philippe, Duangngam Onouma, Kasemsap Poonpipope, Sathornkich Jate, Chayawat Chompunut, Satakhum Duangrat, Priault Pierrick, Desalme Dorine, Chantuma Pisamai, Ghashghaie Jaleh, Epron Daniel. 2016. The source of latex. Tracing carbon from leaf photosynthesis to latex metabolism in rubber trees using carbon stable isotopes. In : CRRI and IRRDB International Rubber Conference 2016, Siem Reap, Cambodia. CRRI, IRRDB. Siem Reap : CRRI-IRRDB, 260-268. International Rubber Conference, Siem Reap, Cambodge, 21 Novembre 2016/25 Novembre 2016.

Résumé : One of the main challenges for the future of Natural Rubber production is the scarcity of skilled manpower to tap the trees. The only way to cope with such issue is to reduce the tapping frequency. The key is the carbon supply to the latex producing tissues. With low tapping frequencies, the latex exported at each tapping day is higher than in traditional systems. Then the trees must mobilize huge amount of carbon at each tapping. Does the latex carbon come directly from the primary sources, the leaves where C is assimilated through photosynthesis, or from reserve pools as wood starch, or both? Knowing the actual C sources and knowing the pathways towards latex is then necessary to manage the tapping systems. Stable isotopes and especially 13C are widely used in plant science as tracers but studying their natural abundance also provides insightful information on tree physiology. We first compared the seasonal variations in natural abundance in tapped and untapped latex of RRIM600 trees to the variations in their leaves which vary according to climate and phenology. We showed that latex δ13C was higher and varied much less than that of leaves in tapped trees. The lack of correlation between variations in δ13C in leaves and in latex suggests that recent photosynthates are mixed in a large pool of stored carbohydrates that are involved in latex regeneration after tapping. We then did a field labeling of full crowns of 3y-old tapped rubber trees with 13CO2 to trace the carbon from its assimilation in the leaves to the tree sinks and particularly to latex. Such experiment, using a specifically designed chamber, has never been done on rubber trees. Three trees (RRIT 408) were labelled in June and three other in October. We sampled leaves, phloem, wood and latex to analyze their 13C content and determine the dynamics of carbon allocation from leaves to reserves and latex. The latex samples will be followed during one year. The presentation describes the methodology and preliminary results showing that the labelling operation was successful.

Classification Agris : F60 - Physiologie et biochimie végétale
F62 - Physiologie végétale - Croissance et développement
U30 - Méthodes de recherche

Auteurs et affiliations

• Thaler Philippe, CIRAD-PERSYST-UMR Eco&Sols (THA) ORCID: 0000-0002-9924-4309
• Duangngam Onouma, Kasetsart University (THA)
• Kasemsap Poonpipope, Kasetsart University (THA)
• Sathornkich Jate, Kasetsart University (THA)
• Chayawat Chompunut, Kasetsart University (THA)
• Satakhum Duangrat, Kasetsart University (THA)
• Priault Pierrick, Université de Lorraine (FRA)
• Desalme Dorine, Université de Lorraine (FRA)
• Chantuma Pisamai, RRIT (THA)
• Ghashghaie Jaleh, CNRS (FRA)
• Epron Daniel, CIRAD-PERSYST-UMR Eco&Sols (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/582928/)

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