Characterization of a large reciprocal chromosomal translocation in banana (Musa acuminata) and its impact on chromosomal segregation using NGS. [W064]

Martin Guillaume, Carreel Françoise, Coriton Olivier, Hervouet Catherine, Cardi Céline, Roques Danièle, Rouard Mathieu, Sardos Julie, Baurens Franc-Christophe, D'Hont Angélique. 2017. Characterization of a large reciprocal chromosomal translocation in banana (Musa acuminata) and its impact on chromosomal segregation using NGS. [W064]. In : Proceedings Plant and Animal Genome XXV Conference. San Diego : PAG, Résumé, 1 p. Plant and Animal Genome Conference. 25, San Diego, États-Unis, 14 Janvier 2017/18 Janvier 2017.

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Résumé : Bananas cultivars are triploid or diploid seedless parthenocarpic clones derived from the hybridization between Musa acuminata subspecies and, for some of them, with the species M. balbisiana. Based on chromosome pairing configuration in hybrids, M. acuminata subspecies were reported to differ by a few large chromosomal structural rearrangements which may have occurred since their divergence following geographical isolation. We analyzed with DArTseq, chromosome segregation in the progenies of a seedy M. acuminata banana accession and discovered strong segregation distortion, reduced recombination and abnormal linkage in two regions of references chromosomes 1 and 4. Pair-end resequencing of the parental accession and comparison to the Musa acuminata references sequence, showed evidence of a heterozygous reciprocal translocation involving two distal segments of 3 Mb and 10 Mb from chromosome 1 and 4. This translocation was then confirmed through BAC-FISH and PCR experiments. The two chromosome structures were found mutually exclusives in gametes and the rearranged structure was transmitted preferentially to the progeny. Analysis of the distribution of this distinct chromosome structures in Musa diversity allowed to make hypotheses about its origin. (Texte intégral)

Classification Agris : F30 - Génétique et amélioration des plantes

Auteurs et affiliations

• Martin Guillaume, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-1801-7500
• Carreel Françoise, CIRAD-BIOS-UMR AGAP (FRA)
• Coriton Olivier, INRA (FRA)
• Hervouet Catherine, CIRAD-BIOS-UMR AGAP (FRA)
• Cardi Céline, CIRAD-BIOS-UMR AGAP (FRA)
• Roques Danièle, CIRAD-BIOS-UMR AGAP (GLP)
• Rouard Mathieu, Bioversity International (FRA)
• Sardos Julie, Bioversity International (FRA)
• Baurens Franc-Christophe, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-5219-8771
• D'Hont Angélique, CIRAD-BIOS-UMR AGAP (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/583216/)

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