Modulation of malaria infection in Anopheles gambiae mosquitoes exposed to natural midgut bacteria

Tchioffo Majoline T., Boissiere Anne, Churcher Thomas S., Abate Luc, Gimonneau Geoffrey, Nsango Sandrine E., Awono-Ambéné Parfait, Christen Richard, Berry Antoine, Morlais Isabelle. 2013. Modulation of malaria infection in Anopheles gambiae mosquitoes exposed to natural midgut bacteria. PloS One, 8 (12):e816663, 9 p.

https://doi.org/10.1371/journal.pone.0081663

Article de revue ; Article de recherche ; Article de revue à facteur d'impact Revue en libre accès total

Prévisualisation

Version publiée - Anglais
Sous licence

.
Tchioffo_2013.PDF
Télécharger (602kB) | Prévisualisation

Url - jeu de données - Entrepôt autre : https://figshare.com/articles/Modulation_of_Malaria_Infection_in_Anopheles_gambiae_Mosquitoes_Exposed_to_Natural_Midgut_Bacteria/871340

Quartile : Outlier, Sujet : MULTIDISCIPLINARY SCIENCES

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie; Staps

Résumé : The development of Plasmodium falciparum within the Anopheles gambiae mosquito relies on complex vector-parasite interactions, however the resident midgut microbiota also plays an important role in mediating parasite infection. In natural conditions, the mosquito microbial flora is diverse, composed of commensal and symbiotic bacteria. We report here the isolation of culturable midgut bacteria from mosquitoes collected in the field in Cameroon and their identification based on the 16S rRNA gene sequencing. We next measured the effect of selected natural bacterial isolates on Plasmodium falciparum infection prevalence and intensity over multiple infectious feedings and found that the bacteria significantly reduced the prevalence and intensity of infection. These results contrast with our previous study where the abundance of Enterobacteriaceae positively correlated with P. falciparum infection (Boissière et al. 2012). The oral infection of bacteria probably led to the disruption of the gut homeostasis and activated immune responses, and this pinpoints the importance of studying microbe-parasite interactions in natural conditions. Our results indicate that the effect of bacterial exposure on P. falciparum infection varies with factors from the parasite and the human host and calls for deeper dissection of these parameters for accurate interpretation of bacterial exposure results in laboratory settings.

Mots-clés Agrovoc : Anopheles gambiae, malaria, Plasmodium falciparum, épidémiologie, interactions biologiques, relation hôte parasite, infection, facteur du milieu, flore intestinale, flore microbienne, Escherichia coli, genre humain, hôte, parasite