Paraburkholderia piptadeniae sp. nov. and Paraburkholderia ribeironis sp. nov., two root-nodulating symbiotic species of Piptadenia gonoacantha in Brazil

Bournaud Caroline, Moulin Lionel, Cnockaert Margo, De Faria Sergio Miana, Prin Yves, Severac Dany, vandamme Pierre. 2017. Paraburkholderia piptadeniae sp. nov. and Paraburkholderia ribeironis sp. nov., two root-nodulating symbiotic species of Piptadenia gonoacantha in Brazil. International Journal of Systematic and Evolutionary Microbiology, 67 (2) : 432-440.

https://doi.org/10.1099/ijsem.0.001648

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Url - jeu de données - Entrepôt autre : https://www.ncbi.nlm.nih.gov/nuccore/LN875219 / Url - jeu de données - Entrepôt autre : https://www.ncbi.nlm.nih.gov/nuccore?term=PRJEB10534

Quartile : Q3, Sujet : MICROBIOLOGY

Note générale : Jeux de données enregistrés dans la base GenBank : The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene, recA and gyrB sequences of P. piptadeniae STM 7183T are LN875219, LN875227 and LN875234 ,respectively, and for the 16S rRNA gene and gyrB sequences of P. ribeironis STM 7296T are LN875221 and LN875236, respectively; accession numbers for the 16S rRNA gene, recA and gyrB sequences of other strains of these two species are given in Fig. 1. The genome sequencing data for P. ribeironis STM 7296T and P. piptadeniae STM 7183T are under project accession numbers PRJEB10534 and PRJEB10535, respectively; with contigs CYGX01000001–CYGX01000228 and CYGY01000001-CYGY01000281, respectively.

Résumé : During a survey of root-nodulating symbionts of Mimosoid species in the south-east region of Brazil, eight Paraburkholderia isolates were obtained from nodules of the legume species Piptadenia gonoacantha, either from the field or following a soil trapping method with the same plant host. 16S rRNA gene as well as recA and gyrB phylogenetic markers placed these strains in two new clades within the genus Burkholderia sensu lato. DNA–DNA hybridization values and analyses of average nucleotide identities of the whole genome sequence of selected strains in each clade (STM 7183 and STM 7296) showed that the two clades represented novel species of the genus Paraburkholderia . All eight isolates were further characterized using DNA base content determination, chemotaxonomic and biochemical profiling and symbiotic properties, which allowed to distinguish the novel species from known diazotrophic species of the genus Paraburkholderia. Based on genomic and phenotypic data, the names Paraburkholderia piptadeniae sp. nov. with type strain STM 7183T (=DSM 101189T=LMG 29163T) and Paraburkholderia ribeironis sp. nov. with type strain STM 7296T (=DSM 101188T=LMG 29351T) are proposed.

Mots-clés géographiques Agrovoc : Brésil

Mots-clés complémentaires : Paraburkholderia, Piptadenia gonoacantha, Paraburkholderia piptadeniae, Paraburkholderia ribeironis

Classification Agris : F30 - Génétique et amélioration des plantes
F60 - Physiologie et biochimie végétale

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

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