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Isolation and full-genome sequences of Japanese encephalitis virus genotype I strains from Cambodian human patients, mosquitoes and pigs

Duong Veasna, Choeung Rithy, Gorman Christopher, Laurent Denis, Crabol Yoann, Mey Channa, Peng Borin, Di Francesco Juliette, Hul Vibol, Sothy Heng, Santy Ky, Richner Beat, Pommier Jean-David, Sorn San, Chevalier Véronique, Buchy Philippe, De Lamballerie Xavier, Cappelle Julien, Horwood Paul, Dussart Philippe. 2017. Isolation and full-genome sequences of Japanese encephalitis virus genotype I strains from Cambodian human patients, mosquitoes and pigs. Journal of General Virology, 98 : pp. 2287-2296.

Journal article ; Article de recherche ; Article de revue à facteur d'impact
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Url - jeu de données : https://www.ncbi.nlm.nih.gov/nuccore/KY927819 / Url - jeu de données : https://www.ncbi.nlm.nih.gov/nuccore/KY927814

Quartile : Q2, Sujet : BIOTECHNOLOGY & APPLIED MICROBIOLOGY / Quartile : Q3, Sujet : VIROLOGY

Additional Information : Jeux de données enregistrés dans la base GenBank : JEV partial NS3 Cambodia: KY927809, KY927810, KY927811, KY927812, KY927813. JEV Envelope Cambodia: KY927814. JEV full genome Cambodia: KY927815, KY927816, KY927817, KY927818, KY927819.

Abstract : Japanese encephalitis remains the most important cause of viral encephalitis in humans in several southeast Asian countries, including Cambodia, causing at least 65 000 cases of encephalitis per year. This vector-borne viral zoonosis – caused by Japanese encephalitis virus (JEV) – is considered to be a rural disease and is transmitted by mosquitoes, with birds and pigs being the natural reservoirs, while humans are accidental hosts. In this study we report the first two JEV isolations in Cambodia from human encephalitis cases from two studies on the aetiology of central nervous system disease, conducted at the two major paediatric hospitals in the country. We also report JEV isolation from Culextritaeniorhynchus mosquitoes and from pig samples collected in two farms, located in peri-urban and rural areas. Out of 11 reverse-transcription polymerase chain reaction-positive original samples, we generated full-genome sequences from 5 JEV isolates. Five additional partial sequences of the JEV NS3 gene from viruses detected in five pigs and one complete coding sequence of the envelope gene of a strain identified in a pig were generated. Phylogenetic analyses revealed that JEV detected in Cambodia belonged to genotype I and clustered in two clades: genotype I-a, mainly comprising strains from Thailand, and genotype I-b, comprising strains from Vietnam that dispersed northwards to China. Finally, in this study, we provide proof that the sequenced JEV strains circulate between pigs, Culex tritaeniorhynchus and humans in the Phnom Penh vicinity. (Résumé d'auteur)

Mots-clés géographiques Agrovoc : Cambodge

Classification Agris : L73 - Animal diseases
000 - Other themes
L72 - Pests of animals

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Agence(s) de financement européenne(s) : European Commission, Directorate-General for Development and Cooperation - EuropeAid

Projet(s) de financement européen(s) : ComAcross project

Auteurs et affiliations

  • Duong Veasna, Institut Pasteur du Cambodge (KHM)
  • Choeung Rithy, Institut Pasteur du Cambodge (KHM)
  • Gorman Christopher, Institut Pasteur du Cambodge (KHM)
  • Laurent Denis, Kantha Bopha Hospital (KHM)
  • Crabol Yoann, Institut Pasteur du Cambodge (KHM)
  • Mey Channa, Institut Pasteur du Cambodge (KHM)
  • Peng Borin, Institut Pasteur du Cambodge (KHM)
  • Di Francesco Juliette, Institut Pasteur du Cambodge (KHM)
  • Hul Vibol, Institut Pasteur du Cambodge (KHM)
  • Sothy Heng, Kantha Bopha Hospital (KHM)
  • Santy Ky, Kantha Bopha Hospital (KHM)
  • Richner Beat, Kantha Bopha Hospital (KHM)
  • Pommier Jean-David, Kantha Bopha Hospital (KHM)
  • Sorn San, NAVRI (KHM)
  • Chevalier Véronique, CIRAD-BIOS-UMR ASTRE (KHM)
  • Buchy Philippe, GlaxoSmithKline (SGP)
  • De Lamballerie Xavier, Université d'Aix-Marseille 2 (FRA)
  • Cappelle Julien, CIRAD-BIOS-UMR ASTRE (FRA) ORCID: 0000-0001-7668-1971
  • Horwood Paul, Institut Pasteur du Cambodge (KHM)
  • Dussart Philippe, Institut Pasteur du Cambodge (KHM)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/585281/)

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