Amblyomma variegatum saliva effects on host immune responses studied by integrative proteomics and cellular biology. [0090]

Holzmuller Philippe, Rodrigues Valérie, Fernandez Bernard, Chamayou Léo, Vercoutere Arthur, Andersen Alexandre, Demettre Edith, Seveno Martial, Vigy Oana, Aprelon Rosalie, Giraud-Girard Ken, Stachurski Frédéric, Vachiéry Nathalie. 2017. Amblyomma variegatum saliva effects on host immune responses studied by integrative proteomics and cellular biology. [0090]. In : Book of abstracts of 9th International conference on tick and tick borne pathogens: One health. TTP9-APRC1. Cairns : TTP9-APRC1, Résumé, 49. 9th International conference on tick and tick borne pathogens, Cairns, Australie, 27 Août 2017/1 Septembre 2017.

Url - éditeur : http://www.ttp9-aprc1.com/

Résumé : Amblyomma variegatum ticks are considered as main pest of ruminants in Africa and in the Caribbean, transmitting heartwater and reactivating bovine dermatophilosis. The objective of our study was to understand the role of Amblyomma saliva in tick-host interactions using proteomics and cellular biology approaches. Among the 336 proteins identified in A. variegatum saliva, a bioinformatics analysis highlighted bioactive molecules with anti-inflammatory and immuno-modulatory properties. We also characterised an ubiquitination complex that could participate in saliva-induced immune suppression of the host. We focused on the immuno-modulating effects of A. variegatum saliva on bovine peripheral blood mononuclear cells (PBMCs) and on macrophages in vitro. We first evidenced a significant decrease of lymphoproliferation for ConA stimulated PBMCs indicative of saliva immuno-suppressive properties. Then, we studied the effect of saliva on bovine macrophages, by measuring nitric oxide production and pro- or anti-inflammatory cytokines such as interleukins IL-10, IL-12p40, and TNF-α. We also analyzed activation and co-stimulatory molecules such as MHC-II, CD40, CD80 or CD86. Finally, we suggest an integrated model of interaction between tick saliva and host immune cells, with a potential effect during tick feeding to favour pathogens dissemination by decreasing host immune response efficacy. Our results bring new insights for a better understanding of tick-ruminant interactions at the molecular level, and pave the way towards integrative strategies to interfere with both the immunosuppressive and infectious processes in corresponding tick-borne diseases. (Texte intégral)

Mots-clés libres : Amblyomma variegatum, Saliva, Proteomics

Classification Agris : L72 - Organismes nuisibles des animaux
L73 - Maladies des animaux

Auteurs et affiliations

• Holzmuller Philippe, CIRAD-BIOS-UMR ASTRE (FRA) ORCID: 0000-0002-8919-9081
• Rodrigues Valérie, CIRAD-BIOS-UMR ASTRE (FRA)
• Fernandez Bernard, CIRAD-BIOS-UMR ASTRE (FRA)
• Chamayou Léo
• Vercoutere Arthur
• Andersen Alexandre
• Demettre Edith, CNRS (FRA)
• Seveno Martial, Institut de génomique fonctionnelle (FRA)
• Vigy Oana, Institut de génomique fonctionnelle (FRA)
• Aprelon Rosalie, CIRAD-BIOS-UMR ASTRE (GLP)
• Giraud-Girard Ken, CIRAD-BIOS-UMR ASTRE (GLP)
• Stachurski Frédéric, CIRAD-BIOS-UMR ASTRE (FRA) ORCID: 0009-0009-5904-3375
• Vachiéry Nathalie, CIRAD-BIOS-UMR ASTRE (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/586520/)

Métriques

[ Page générée et mise en cache le 2024-12-18 ]