Presence of 16SrIV phytoplasmas of subgroups A,D and E in planthopper Haplaxius crudus Van Duzee insects in Yucatan, Mexico

Narvaez Maria, Vázquez-Euán Roberto, Harrison Nigel, Nic-Matos Germán, Julia Jean-François, Dzido Jean-Luc, Fabre Sandrine, Dollet Michel, Oropeza Carlos. 2018. Presence of 16SrIV phytoplasmas of subgroups A,D and E in planthopper Haplaxius crudus Van Duzee insects in Yucatan, Mexico. 3 Biotech, 8 (1):61, 9 p.

https://doi.org/10.1007/s13205-018-1094-5

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Quartile : Q3, Sujet : BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Résumé : The present study was carried out to determine if group 16SrIV phytoplasmas, causing lethal yellowing (LY) disease, are present in Haplaxius crudus Van Duzee (Hemiptera: Cixiidae) insects associated with palms in Yucatán, Mexico. Haplaxius crudus feral insects were captured from palm foliage at two locations (Chicxulub Puerto and CICY, Mérida, where LY-type diseases are active) and evaluated individually for the presence of phytoplasma DNA by a group 16SrIV-specific nested PCR assay. The results showed positive detection in H. crudus insects in a proportion of 2.7% (of the total 2726 analyzed) during a 3-year period of study. The percentage of detection was different for each site, 5.9% positive of 799 insects from Mérida and 1.7% of 1927 from Chicxulub Puerto. Positive detections were also obtained in extracts from 5.3 to 1.2% of males and females, respectively. Sequencing and in silico RFLP and phylogenetic analyses of PCR-amplified rDNA products indicated that H. crudus insects from Chicxulub Puerto harbored phytoplasma strains of subgroups 16SrIV-A or 16SrIV-D, whereas in insects from Mérida the strains found were 16SrIV-A, 16SrIV-D or 16SrIV-E. The diversity of subgroup strains detected in H. crudus coincided with strains previously identified in palms showing LY-type disease syndromes in Yucatán thereby implicating H. crudus as a candidate vector of 16SrIV phytoplasmas in this region of Mexico.

Mots-clés Agrovoc : Cixiidae, vecteur de maladie, phytoplasme, transmission des maladies, maladie bactérienne, identification, PCR, Arecaceae, Cocos nucifera, ravageur des plantes

Mots-clés géographiques Agrovoc : Yucatan

Mots-clés complémentaires : Haplaxius crudus, Jaunissement mortel

Mots-clés libres : Lethal yellowing-type syndromes, Haplaxius crudus, Phytoplasmas, PCR detection, Transmission, Vecteur

Classification Agris : H10 - Ravageurs des plantes
H20 - Maladies des plantes

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

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