Spatial genetic structure of Xanthomonas citri pv. citri at microgeographical scales through culture-independent microsatellite genotyping in a tropical disease endemicity setting

Pruvost Olivier, Boyer Karine, Ravigné Virginie, Richard Damien, Rieux Adrien, Lefeuvre Pierre, Vernière Christian. 2018. Spatial genetic structure of Xanthomonas citri pv. citri at microgeographical scales through culture-independent microsatellite genotyping in a tropical disease endemicity setting. In : 13èmes Rencontres Plantes-Bactéries - Book of Abstracts. SFP, INRA, CNRS. Paris : SFP, Résumé, 114. Rencontres Plantes-Bactéries. 13, Aussois, France, 29 Janvier 2018/2 Février 2018.

Matériel d'accompagnement : 1 poster

Résumé : Population biology has the ability to refine our understanding of the biology of plant pathogenic xanthomonads, which remains partial despite the major significance of some of them as agricultural threats. We developed direct microsatellite genotyping from canker lesions caused by Xanthomonas citri pv. citri and applied it to investigate the genetic and spatial structure of the pathogen at several microgeographic scales in a situation of disease endemicity. This approach allowed identifying outbreaks with multiple inoculum sources and comparing genetic lineages in terms of transmission ability. ln a citrus grove where copper-susceptible and copper-resistant strains co-existed, our results suggested a range of spatial dependencyfourtimes largerforthe latter ones. Aggregated spatial patterns estimated from genotyping data were consistent with that previously determined from disease severity data in Argentina. How RST outperformed FST for differentiation analyses was scale-dependent. Linkage disequilibrium was maintained at smalt scales, likely as a confounding consequence of clonality and admixture. Finally, our results highlighted in ca. 1 % of samples a within-lesion polymorphism that likely reflects polyclonal infections. Because of their major biological significance as an inoculum source, we suggest that cankers may represent a preferred niche for horizontal gene transfer.

Classification Agris : H20 - Maladies des plantes

Auteurs et affiliations

• Pruvost Olivier, CIRAD-BIOS-UMR PVBMT (REU)
• Boyer Karine, CIRAD-BIOS-UMR PVBMT (REU)
• Ravigné Virginie, CIRAD-BIOS-UMR PVBMT (REU) ORCID: 0000-0002-4252-2574
• Richard Damien, CIRAD-BIOS-UMR PVBMT (REU)
• Rieux Adrien, CIRAD-BIOS-UMR PVBMT (REU)
• Lefeuvre Pierre, CIRAD-BIOS-UMR PVBMT (REU)
• Vernière Christian, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0002-2312-2073

Source : Cirad-Agritrop (https://agritrop.cirad.fr/587100/)

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