Genome-based survey of non-ribosomal peptide synthetase genes sharing fascinating features with the albicidin biosynthesis genes

Sabri Souhir, Royer Monique, Pesic Alexander, Petras Daniel, Mainz Andi, Süssmuth Roderich, Cociancich Stéphane. 2018. Genome-based survey of non-ribosomal peptide synthetase genes sharing fascinating features with the albicidin biosynthesis genes. . INRA. Aussois : INRA, Résumé, p. 70. Rencontres Plantes-Bactéries. 13, Aussois, France, 29 Janvier 2018/2 Février 2018.

Résumé : Albicidin and cystobactamid are potent DNA gyrase inhibitors exhibiting very similar structures [1,2]. They are produced by the sugarcane pathogenic bacterium Xanthomonas albilineans and the soil bacterium Cystobacter spp., respectively. Their biosynthesis involves identical families of NRPS genes that have never been described to date in any other biosynthesis pathway. For example, adenylation domains exhibiting unusual specificity - conferring signatures activate para-aminobenzoate (p ABA). Likewise, an asparagine-activating adenylation domain exhibiting an unusual 342-amino-acid insertion which harbors the ATP-binding motif SGGKD was proposed to be linked to the incorporation of cyanoalanine in albicidin. Non-ribosomal peptide synthetases (NRPS) systems constitute a yet underexplored source of natural products, but genomics -based approaches open up new opportunities for a more efficient and rational exploration. A genome -based survey was performed with bacterial sequences available on GenBank in order to identify new NRPS loci sharing features with albicidin and cystobactamid loci. As a result, 13 different loci encoding an adenylation domain containing the above-described 342-amino-acid insertion were identified in the genera Arenibacter, Algoriphagus, Candidatus, Clostridium, Leptolyngbya, Microvirga, Paenibacillus, Roseobacter, Ruegeria, Verrucomicrobiae and Zobellia, respectively. Additionally, three different hitherto unknown NRPS loci encoding a pABA-activating adenylation domain were identified in the genera Actinoplanes, Janthinobacterium and Xanthomonas, respectively. Comparative analyses provide in silico insights on the functional organization of these newly discovered NRPS domains. Additionally, annotation of the unknown NRPS loci identified in this study indicates that they are involved in biosynthesis of entirely different molecules and are therefore promising candidates for further experimental studies aiming at discovering new natural products.

Classification Agris : 000 - Autres thèmes
H20 - Maladies des plantes

Auteurs et affiliations

• Sabri Souhir
• Royer Monique, CIRAD-BIOS-UMR BGPI (FRA)
• Pesic Alexander, Technische Universitaet Berlin (DEU)
• Petras Daniel, Technische Universitaet Berlin (DEU)
• Mainz Andi, Technische Universitaet Berlin (DEU)
• Süssmuth Roderich, Technische Universitaet Berlin (DEU)
• Cociancich Stéphane, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0001-8601-3598

Source : Cirad-Agritrop (https://agritrop.cirad.fr/587120/)

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