Diagnostics through Ralstonia solanacearum genomics: grasping the broad diversity of a complex plant pathogen. [S4-O4]

Cellier Gilles, Ailloud Florent, Hostachy Bruno, Prior Philippe, Robène-Soustrade Isabelle. 2014. Diagnostics through Ralstonia solanacearum genomics: grasping the broad diversity of a complex plant pathogen. [S4-O4]. In : The 13th International Conference on Plant Pathogenic Bacteria. Programme and abstracts. SCPBD. Shanghai : SCPBD, Résumé, 93. 13th International Conference on Plant Pathogenic Bacteria, Shanghai, Chine, 8 Juin 2014/13 Juin 2014.

Résumé : designed to identify 17 phylogenetic clusters of diagnostic interest, including species, phylotype or sequevar-specific probes along with control probe . Highlight was given on three ecotypes in the phylotype II: (i) Brown rot trains from sequevars IIB-1 ; (ii) Moko disease-causing srains from sequevars IIB-3 IIB-4, IIA-6 and IIA-24, that cause wilt on banana plantain, and Heliconia; and (iii) new pathogenic variants from sequevar IIB-4NPB that lacks pathogenicity to banana Cavendish but can infect many other plant species, including Cucurbitacae. As a first step, a spotted array glass slide format was chosen for its probe screening capacity, where the 100 best 50mers probes out of more than l 000 were selected on 80 strains for switching to a more convenient microarray format, based on the "Array Tube" technology from Alere Technologies, which allows micro-reactions within a vial. Several advantages to witch to this technology will be presented along with first results.

Classification Agris : H20 - Maladies des plantes

Auteurs et affiliations

• Cellier Gilles, ANSES-LSV (REU)
• Ailloud Florent, ANSES-LSV (REU)
• Hostachy Bruno, ANSES-LSV (REU)
• Prior Philippe, CIRAD-BIOS-UMR PVBMT (REU)
• Robène-Soustrade Isabelle, CIRAD-BIOS-UMR PVBMT (REU)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/587149/)

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