CRISPR systems in plant pathogens: A new tool for epidemiological surveillance

Jeong Kwanho, Munoz Bodnar Alejandra, Poulin Lucie, Arias Rojas Nathalia, Rodríguez-R Luis Miguel, Gagnevin Lionel, Pruvost Olivier, Koebnik Ralf. 2013. CRISPR systems in plant pathogens: A new tool for epidemiological surveillance. Phytopathology, 103 (6), suppl. S2 : S2.67-S2.68. APS-MSA Conference, Austin, États-Unis, 10 Août 2013/14 Août 2013.

Quartile : Q1, Sujet : PLANT SCIENCES

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie

Résumé : Rapidly evolving genetic loci, such as Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR), are of special interest to develop new molecular typing tools. The widely distributed CRISPR loci consist of highly conserved DNA repeats that are interspersed by unique, similarly sized spacers which originate from previous attacks by viruses and/or plasmids. For efficient management of plant diseases, knowledge about the pathogen's population structure and tools for epidemiological surveillance are prerequisite. We wish to exploit CRISPR loci as a molecular typing tool of plant pathogens, as exemplified by Xanthomonas, an important clade of Gramnegative bacteria infecting a plethora of plants, including rice, cereals, cassava, banana and citrus fruits. CRISPR loci of 56 X. citri subsp. citri strains of world-wide origin were sequenced, revealing a repertoire of 37 unique spacers. A dendrogram based on the presence and absence of spacers was largely congruent with previous typing using AFLP. Our results demonstrate that CRISPR-based spoligotyping can be used as an efficient and robust method to study the phylogenetic relationships among isolates of plantpathogenic xanthomonads, such as the citrus cancer pathogen X. citri subsp. citri. Implementation of Xanthomonas spoligotyping will serve phytosanitary measures by assisting the epidemiological surveillance of outbreaks of citrus canker and other diseases. (Texte intégral)

Classification Agris : H20 - Maladies des plantes
C30 - Documentation et information

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Jeong Kwanho, IRD (FRA)
• Munoz Bodnar Alejandra, IRD (FRA)
• Poulin Lucie, IRD (FRA)
• Arias Rojas Nathalia, IRD (FRA)
• Rodríguez-R Luis Miguel, IRD (FRA)
• Gagnevin Lionel, CIRAD-BIOS-UMR PVBMT (REU)
• Pruvost Olivier, CIRAD-BIOS-UMR PVBMT (REU)
• Koebnik Ralf, IRD (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/587407/)

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