Molecular identification of Anaplasma marginale in two autochthonous South American wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines

Guillemi Eliana C., De la Fourniere Sofía, Orozco Marcela, Martinez Jorge Peña, Correa Elena, Fernandez Javier, Lopez Arias Ludmila, Paoletta Martina, Corona Belkis, Pinarello Valérie, Wilkowsky Silvina E., Farber Marisa D.. 2016. Molecular identification of Anaplasma marginale in two autochthonous South American wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines. Parasites and Vectors, 9:358, 9 p.

Quartile : Q1, Sujet : PARASITOLOGY

Note générale : Corrigendum paru dans Parasites and Vectors, (2016)1 p. https://doi.org/10.1186/s13071-016-1653-8

Résumé : Background: Anaplasma marginale is a well-known cattle pathogen of tropical and subtropical world regions. Even though, this obligate intracellular bacterium has been reported in other host species different than bovine, it has never been documented in Myrmecophaga tridactyla (giant anteater) or Hippocamelus antisense (taruca), which are two native endangered species. Methods: Samples from two sick wild animals: a Myrmecophaga tridactyla (blood) and a Hippocamelus antisense (blood and serum) were studied for the presence of A. marginale DNA through msp5 gene fragment amplification. Further characterization was done through MSP1a tandem repeats analysis and MLST scheme and the genetic relationship among previously characterized A. marginale sequences were studied by applying, eBURST algorithm and AMOVA analysis. Results: Anaplasma marginale DNA was identified in the Myrmecophaga tridactyla and Hippocamelus antisense samples. Through molecular markers, we identified an identical genotype in both animals that was not previously reported in bovine host. The analysis through eBURST and AMOVA revealed no differentiation between the taruca/anteater isolate and the bovine group. Conclusions: In the present publication we report the identification of A. marginale DNA in a novel ruminant (Hippocamelus antisense) and non-ruminant (Myrmecophaga tridactyla) host species. Genotyping analysis of isolates demonstrated the close relatedness of the new isolate with the circulation population of A. marginale in livestock. Further analysis is needed to understand whether these two hosts contribute to the anaplasmosis epidemiology.

Mots-clés géographiques Agrovoc : Argentine

Classification Agris : L73 - Maladies des animaux
L20 - Écologie animale
P01 - Conservation de la nature et ressources foncières

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

• Guillemi Eliana C., INTA (ARG) - auteur correspondant
• De la Fourniere Sofía, INTA (ARG)
• Orozco Marcela, UBA (ARG)
• Martinez Jorge Peña, Conservation Land Trust (ARG)
• Correa Elena, Universidad Nacional de Tucuman (ARG)
• Fernandez Javier, Universidad Nacional de Tucuman (ARG)
• Lopez Arias Ludmila, INTA (ARG)
• Paoletta Martina, INTA (ARG)
• Corona Belkis, National Center of Animal and Plant Health (CUB)
• Pinarello Valérie, CIRAD-BIOS-UPR Contrôle des maladies (GLP) ORCID: 0000-0002-9209-2111
• Wilkowsky Silvina E., INTA (ARG)
• Farber Marisa D., INTA (ARG)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/587974/)

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