Transcriptome analysis in Hevea brasiliensis latex revealed changes in hormone signalling pathways during ethephon stimulation and consequent Tapping Panel Dryness

Montoro Pascal, Wu Shuangyang, Favreau Bénédicte, Herlinawati Eva, Labrune Cécile, Martin-Magniette Marie-Laure, Pointet Stéphanie, Rio Maryannick, Leclercq Julie, Ismawanto Sigit, Kuswanhadi. 2018. Transcriptome analysis in Hevea brasiliensis latex revealed changes in hormone signalling pathways during ethephon stimulation and consequent Tapping Panel Dryness. Scientific Reports, 8:8483, 12 p.

https://doi.org/10.1038/s41598-018-26854-y

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Url - jeu de données - Entrepôt autre : https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE101568

Quartile : Q1, Sujet : MULTIDISCIPLINARY SCIENCES

Note générale : The RNA sequencing data have been submitted to the international repository GEO (Gene Expression Omnibus, Edgard R. et al. 2002, http://www.ncbi.nlm.nih.gov/geo) ProjectID GSE101568

Résumé : Tapping Panel Dryness (TPD) affects latex production in Hevea brasiliensis. This physiological syndrome involves the agglutination of rubber particles, which leads to partial or complete cessation of latex flow. Latex harvesting consists in tapping soft bark. Ethephon can be applied to stimulate latex flow and its regeneration in laticifers. Several studies have reported transcriptome changes in bark tissues. This study is the first report on deep RNA sequencing of latex to compare the effect of ethephon stimulation and TPD severity. Trees were carefully selected for paired-end sequencing using an Illumina HiSeq 2000. In all, 43 to 60 million reads were sequenced for each treatment in three biological replicates (slight TPD trees without ethephon stimulation, and slight and severe TPD trees with ethephon treatment). Differentially expressed genes were identified and annotated, giving 8,111 and 728 in response to ethephon in slight TPD trees and in ethephon-induced severe TPD trees, respectively. A biological network of responses to ethephon and TPD highlighted the major influence of metabolic processes and the response to stimulus, especially wounding and jasmonate depression in TPD-affected trees induced by ethephon stimulation.

Mots-clés Agrovoc : Hevea brasiliensis, latex, caoutchouc, éthylène, jasmonate, arn, saignée, expression des gènes

Mots-clés géographiques Agrovoc : Indonésie

Mots-clés libres : Ethylene, Jasmonate, Laticifer, RNA, Rubber, Sequencing

Classification Agris : F60 - Physiologie et biochimie végétale
F30 - Génétique et amélioration des plantes
F01 - Culture des plantes

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

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