Transcriptome analysis in Hevea brasiliensis latex revealed changes in hormone signalling pathways during ethephon stimulation and consequent Tapping Panel Dryness

Montoro Pascal, Wu Shuangyang, Favreau Bénédicte, Herlinawati Eva, Labrune Cécile, Martin-Magniette Marie-Laure, Pointet Stéphanie, Rio Maryannick, Leclercq Julie, Ismawanto Sigit, Kuswanhadi. 2018. Transcriptome analysis in Hevea brasiliensis latex revealed changes in hormone signalling pathways during ethephon stimulation and consequent Tapping Panel Dryness. Scientific Reports, 8:8483, 12 p.

Journal article ; Article de recherche ; Article de revue à facteur d'impact Revue en libre accès total
Published version - Anglais
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Additional Information : The RNA sequencing data have been submitted to the international repository GEO (Gene Expression Omnibus, Edgard R. et al. 2002, ProjectID GSE101568

Abstract : Tapping Panel Dryness (TPD) affects latex production in Hevea brasiliensis. This physiological syndrome involves the agglutination of rubber particles, which leads to partial or complete cessation of latex flow. Latex harvesting consists in tapping soft bark. Ethephon can be applied to stimulate latex flow and its regeneration in laticifers. Several studies have reported transcriptome changes in bark tissues. This study is the first report on deep RNA sequencing of latex to compare the effect of ethephon stimulation and TPD severity. Trees were carefully selected for paired-end sequencing using an Illumina HiSeq 2000. In all, 43 to 60 million reads were sequenced for each treatment in three biological replicates (slight TPD trees without ethephon stimulation, and slight and severe TPD trees with ethephon treatment). Differentially expressed genes were identified and annotated, giving 8,111 and 728 in response to ethephon in slight TPD trees and in ethephon-induced severe TPD trees, respectively. A biological network of responses to ethephon and TPD highlighted the major influence of metabolic processes and the response to stimulus, especially wounding and jasmonate depression in TPD-affected trees induced by ethephon stimulation.

Mots-clés Agrovoc : Hevea brasiliensis, Latex, Caoutchouc, Éthylène, Jasmonate, ARN, Saignée, Expression des gènes

Mots-clés géographiques Agrovoc : Indonésie

Mots-clés libres : Ethylene, Jasmonate, Laticifer, RNA, Rubber, Sequencing

Classification Agris : F60 - Plant physiology and biochemistry
F30 - Plant genetics and breeding
F01 - Crop husbandry

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

Auteurs et affiliations

  • Montoro Pascal, CIRAD-BIOS-UMR AGAP (FRA) - auteur correspondant
  • Wu Shuangyang, CIRAD-BIOS-UMR AGAP (FRA)
  • Favreau Bénédicte, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0003-2786-9374
  • Herlinawati Eva, IRRI (IDN)
  • Labrune Cécile, CNRS (FRA)
  • Martin-Magniette Marie-Laure, CNRS (FRA)
  • Pointet Stéphanie, Université de Montpellier (FRA)
  • Rio Maryannick, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-4573-1371
  • Leclercq Julie, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-8110-3762
  • Ismawanto Sigit, IRRI (IDN)
  • Kuswanhadi, Indonesian Rubber Research Institute (IDN)

Source : Cirad-Agritrop (

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