Genetic effect in leaf and xylem transcriptome variations among Eucalyptus urophylla x grandis hybrids in field conditions

Vaillant Alexandre, Honvault Astrid, Bocs Stéphanie, Summo Marilyne, Makouanzi Garel, Vigneron Philippe, Bouvet Jean-Marc. 2018. Genetic effect in leaf and xylem transcriptome variations among Eucalyptus urophylla x grandis hybrids in field conditions. Silvae Genetica, 67 (1) : 57-65.

Url - autre : https://www.degruyter.com/view/j/sg.2018.67.issue-1/sg-2018-0008/suppl/sg-2018-0008_sm.zip

Quartile : Q4, Sujet : FORESTRY / Quartile : Q4, Sujet : GENETICS & HEREDITY

Résumé : To assess the genetic and environmental components of gene-expression variation among trees we used RNA-seq technology and Eucalyptus urophylla x grandis hybrid clones tested in field conditions. Leaf and xylem transcriptomes of three 20 month old clones differing in terms of growth, repeated in two blocks, were investigated. Transcriptomes were very similar between ramets. The number of expressed genes was significantly (P<0.05) higher in leaf (25,665±634) than in xylem (23,637±1,241). A pairwise clone comparisons approach showed that 4.5 to 14 % of the genes were diffe¬rentially expressed (false discovery rate [FDR]<0.05) in leaf and 7.1 to 16 % in xylem. An assessment of among clone variance components revealed significant results in leaf and xylem in 3431 (248) genes (at FDR<0.2) and 160 (3) (at FDR<0.05), respectively. These two complementary approa¬ches displayed correlated results. A focus on the phenylpro¬panoid, cellulose and xylan pathways revealed a large majo¬rity of low expressed genes and a few highly expressed ones, with RPKM values ranging from nearly 0 to 600 in leaf and 10,000 in xylem. Out of the 115 genes of these pathways, 45 showed differential expression for at least one pair of geno¬type, five of which displaying also clone variance compo¬nents. These preliminary results are promising in evaluating whether gene expression can serve as possible 'intermediate phenotypes' that could improve the accuracy of selection of grossly observable traits.

Mots-clés libres : Forest tree, Eucalyptus, Gene differential expression, Genetic control, Lignin, Cellulose

Classification Agris : K50 - Technologie des produits forestiers
F50 - Anatomie et morphologie des plantes
F30 - Génétique et amélioration des plantes

Champ stratégique Cirad : Axe 2 (2014-2018) - Valorisation de la biomasse

Auteurs et affiliations

• Vaillant Alexandre, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-4785-1350 - auteur correspondant
• Honvault Astrid, INRA (FRA)
• Bocs Stéphanie, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-7850-4426
• Summo Marilyne, CIRAD-BIOS-UMR AGAP (FRA)
• Makouanzi Garel, CRDPI (COG)
• Vigneron Philippe, CIRAD-ES-UPR Forêts et sociétés (FRA)
• Bouvet Jean-Marc, CIRAD-BIOS-UMR AGAP (MDG)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/588409/)

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