Detection and quantification of ochratoxin A in food by aptasensor

Elsaadani Moez, Durand Noel, Sorli Brice, Montet Didier. 2018. Detection and quantification of ochratoxin A in food by aptasensor. In : 12th Conference RME2018: Rapid analysis and diagnostics. Food feed water animal human. Abstracts of lecutres and posters. Bilthoven : Bastiaanse Communication, Résumé, 74-75. (RME Conference Series, 12) Conference in the Rapid Methods Europe (RME 2018). 12, Amsterdam, Pays-Bas, 5 Novembre 2018/7 Novembre 2018.

Matériel d'accompagnement : 1 poster

Note générale : Titre du poster : A label free aptasensor for ochratoxin A detection

Résumé : Governments and international instances are trying to improve the food safety system to prevent, reduce or avoid the increase of food borne diseases. This food risk is one of the major concerns for the humanity. The contamination by mycotoxins is a threat to the health and life of humans and animals. One of the most common mycotoxin contaminating feed and foodstuffs is ochratoxin A (OTA), which is a secondary metabolite, produced by Aspergillus and Penicillium strains. OTA has a chronic toxic effect and proved to be mutagenic, nephrotoxic, teratogenic, immunosuppressive, and carcinogenic. On the other side, because of their high stability, specificity, affinity, and their easy chemical synthesis, aptamerbased methods are applied to OTA biosensing as alternative to traditional analytical technique. In this work, five aptamers have been tested to confirm qualitatively and quantitatively their binding with OTA. In the same time, three different analytical methods were tested and compared based on their ability to detect and quantify the OTA. The best protocol that was established to quantify free OTA from linked OTA involved an ultrafiltration method in green coffee solution with. OTA was quantified by HPLC-FLD to calculate the binding percentage of all five aptamers. One aptamer (The most effective with 87% binding with OTA) has been selected to be our biorecognition element to study its electrical response (variation of electrical properties) in the presence of OTA in order to be able to make a pairing with Radio Frequency Identification (RFID). This device, which is characterised by its low cost, speed and a simple wireless information transmission, will implement the knowledge on the mycotoxins molecular sensors (aptamers), an electronic device that will link the information, the quantification and make it available to operators.

Mots-clés libres : Ochratoxin A, Aptamer, Label free Aptasensor, Detection

Auteurs et affiliations

• Elsaadani Moez, Université de Montpellier (FRA)
• Durand Noel, CIRAD-PERSYST-UMR Qualisud (FRA) ORCID: 0000-0003-1627-6848
• Sorli Brice, Université de Montpellier (FRA)
• Montet Didier, CIRAD-PERSYST-UMR Qualisud (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/589476/)

Métriques

[ Page générée et mise en cache le 2023-06-24 ]