Biosynthetic investigation of γ-lactones in Sextonia rubra wood using in situ TOF-SIMS MS/MS imaging to localize and characterize biosynthetic intermediates

Fu Tingting, Houël Emeline, Amusant Nadine, Touboul David, Genta-Jouve Grégory, Della Negra Serge, Fisher Gregory L., Brunelle Alain, Duplais Christophe. 2019. Biosynthetic investigation of γ-lactones in Sextonia rubra wood using in situ TOF-SIMS MS/MS imaging to localize and characterize biosynthetic intermediates. Scientific Reports, 9:1928, 11 p.

https://doi.org/10.1038/s41598-018-37577-5

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Quartile : Q1, Sujet : MULTIDISCIPLINARY SCIENCES

Résumé : Molecular analysis by parallel tandem mass spectrometry (MS/MS) imaging contributes to the in situ characterization of biosynthetic intermediates which is crucial for deciphering the metabolic pathways in living organisms. We report the first use of TOF-SIMS MS/MS imaging for the cellular localization and characterization of biosynthetic intermediates of bioactive γ-lactones rubrynolide and rubrenolide in the Amazonian tree Sextonia rubra (Lauraceae). Five γ-lactones, including previously reported rubrynolide and rubrenolide, were isolated using a conventional approach and their structural characterization and localization at a lateral resolution of ~400 nm was later achieved using TOF-SIMS MS/MS imaging analysis. 2D/3D MS imaging at subcellular level reveals that putative biosynthetic γ-lactones intermediates are localized in the same cell types (ray parenchyma cells and oil cells) as rubrynolide and rubrenolide. Consequently, a revised metabolic pathway of rubrynolide was proposed, which involves the reaction between 2-hydroxysuccinic acid and 3-oxotetradecanoic acid, contrary to previous studies suggesting a single polyketide precursor. Our results provide insights into plant metabolite production in wood tissues and, overall, demonstrate that combining high spatial resolution TOF-SIMS imaging and MS/MS structural characterization offers new opportunities for studying molecular and cellular biochemistry in plants.

Mots-clés Agrovoc : bois, composition chimique

Mots-clés géographiques Agrovoc : Guyane française, France

Mots-clés complémentaires : Sextonia rubra

Classification Agris : K50 - Technologie des produits forestiers
F60 - Physiologie et biochimie végétale

Champ stratégique Cirad : CTS 7 (2019-) - Hors champs stratégiques

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