Genome-wide diversity and gene expression profiling of Babesia microti isolates identify polymorphic genes that mediate host-pathogen interactions

Silva Joana C., Cornillot Emmanuel, McCracken Carrie, Usmani-Brown Sahar, Dwivedi Ankit, Ifeonu Olukemi O., Crabtree Jonathan, Gotia Hanzel T., Virji Azan Z., Reynes Christelle, Colinge Jacques, Kumar Vidya, Lawres Lauren, Pazzi Joseph E., Pablo Jozelyn V., Hung Chris, Brancato Jana, Kumari Priti, Orvis Joshua, Tretina Kyle, Chibucos Marcus, Ott Sandy, Sadzewicz Lisa, Sengamalay Naomi, Shetty Amol C., Su Qi, Tallon Luke, Fraser Claire M., Frutos Roger, Molina Douglas M., Krause Peter J., Ben Mamoun Choukri. 2016. Genome-wide diversity and gene expression profiling of Babesia microti isolates identify polymorphic genes that mediate host-pathogen interactions. Scientific Reports, 6:35284, 15 p.

https://doi.org/10.1038/srep35284

Article de revue ; Article de recherche ; Article de revue à facteur d'impact Revue en libre accès total

Version publiée - Anglais
Sous licence

.
Genome Babesia.pdf
Télécharger (2MB)

Quartile : Q1, Sujet : MULTIDISCIPLINARY SCIENCES

Résumé : Babesia microti, a tick-transmitted, intraerythrocytic protozoan parasite circulating mainly among small mammals, is the primary cause of human babesiosis. While most cases are transmitted by Ixodes ticks, the disease may also be transmitted through blood transfusion and perinatally. A comprehensive analysis of genome composition, genetic diversity, and gene expression profiling of seven B. microti isolates revealed that genetic variation in isolates from the Northeast United States is almost exclusively associated with genes encoding the surface proteome and secretome of the parasite. Furthermore, we found that polymorphism is restricted to a small number of genes, which are highly expressed during infection. In order to identify pathogen-encoded factors involved in host-parasite interactions, we screened a proteome array comprised of 174 B. microti proteins, including several predicted members of the parasite secretome. Using this immuno-proteomic approach we identified several novel antigens that trigger strong host immune responses during the onset of infection. The genomic and immunological data presented herein provide the first insights into the determinants of B. microti interaction with its mammalian hosts and their relevance for understanding the selective pressures acting on parasite evolution.

Mots-clés Agrovoc : Babesia microti, expression des gènes, variation génétique, relation hôte pathogène, Ixodes, sang

Mots-clés géographiques Agrovoc : États-Unis d'Amérique

Classification Agris : L73 - Maladies des animaux
000 - Autres thèmes

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations