Large chromosomal structural variations between M. acuminata and M balbisiana and their consequences on chromosome recombination and segregation in a AAAB polyploidy context

Baurens Franc-Christophe, Martin Guillaume, Rouard Mathieu, Salmon Frédéric, Njembele J. Célestin, Habas Rémy, Ricci Sébastien, D'Hont Angélique. 2017. Large chromosomal structural variations between M. acuminata and M balbisiana and their consequences on chromosome recombination and segregation in a AAAB polyploidy context. In : Abstract Book Dynagev 2017. Réseau scientifique DYNAGEV. Montpellier : INRA, Résumé, 20. Colloque annuel DynaGeV : Dynamique des génomes végétaux. 3, Montpellier, France, 8 Juin 2017/9 Juin 2017.

Résumé : Many banana cultivars are triploid interspecific hybrids between M. acuminata (Genome A, 2n=22) and M. balbisiana (Genome B, 2n=22). They included the important group of Plantain cooking bananas classified as AAB. We compared the global chromosome structure of A and B genomes through the construction of a M. balbisiana genetic map and its comparison with the M. acuminata reference sequence assembly. We identified a large reciprocal translocation involving chromosome 1 and 3 and a large inversion on chromosome 5. We analyzed the A/B chromosomes composition of plantain cultivars revealing a few chromosome segments with AAA composition and one entire chromosome with ABB composition instead of the supposed general 'AAB' composition. We analyzed the A/B chromosomes composition of a progeny from a 'AAAB' tetraploid plantain derived breeding accession. We observed recombination between A and B genomes along most of the chromosomes. A few exceptions were observed with no recombination in the inverted segment between A and B on chromosome 5 and a reduced recombination near the translocated breakpoints on chromosome 1 and 3. We also observed a very important number of aneuploids in the progeny (62%) with 6% involving chromosome 5 and 46% involving chromosome 1 and 3, the three chromosomes that differed in their global structure between A and B genomes. Implication of these results on the origin of banana cultivars and on breeding of banana allopolyploids will be discussed.

Auteurs et affiliations

• Baurens Franc-Christophe, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-5219-8771
• Martin Guillaume, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-1801-7500
• Rouard Mathieu, Bioversity International (FRA)
• Salmon Frédéric, CIRAD-BIOS-UMR AGAP (GLP)
• Njembele J. Célestin, CARBAP (CMR)
• Habas Rémy, CIRAD-BIOS-UMR BGPI (FRA)
• Ricci Sébastien, CIRAD-BIOS-UMR AGAP (GLP) ORCID: 0000-0001-7189-9592
• D'Hont Angélique, CIRAD-BIOS-UMR AGAP (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/592914/)

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