Development and validation of a pen side test for Rift Valley fever

Cetre-Sossah Catherine, Pédarrieu Aurélie, Juremalm Mikael, Jansen Van Vuren Petrus, Brun Alejandro, Ould El Mamy Ahmed Bezeid, Héraud Jean Michel, Filippone Claudia, Ravalohery Jean-Pierre, Chaabihi Hassan, Albina Emmanuel, Dommergues Laure, Paweska Janusz, Cardinale Eric. 2019. Development and validation of a pen side test for Rift Valley fever. PLoS Neglected Tropical Diseases, 13 (9):e0007700, 16 p.

https://doi.org/10.1371/journal.pntd.0007700

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Quartile : Q1, Sujet : TROPICAL MEDICINE / Quartile : Q1, Sujet : PARASITOLOGY

Résumé : Background: Rift Valley fever (RVF) is one of the main vector borne zoonotic diseases that affects a wide range of ruminants and human beings in Africa and the Arabian Peninsula. A rapid and specific test for RVF diagnosis at the site of a suspected outbreak is crucial for the implementation of control measures. Methodology/Principal findings: A first-line lateral flow immunochromatographic strip test (LFT) was developed for the detection of the nucleoprotein (N) of the RVF virus (RVFV). Its diagnostic performance characteristics were evaluated using reference stocks isolates recovered from different hosts and in geographic regions mimicking clinical specimens and from known RVF negative serum samples. A high level of diagnostic accuracy (DSe (35/35), DSp (167/169)) was observed, including the absence of cross-reactivity with viruses belonging to different genera. Conclusion/Significance: The fact no specialized reagents and laboratory equipment are needed, make this assay a valuable, first-line diagnostic tool in resource-poor diagnostic territories for on-site RVFV detection, however the staff require training. Author: summary: Rift Valley fever (RVF) is a viral disease that affects a wide range of animals and human beings in Africa and the Arabian Peninsula involving low case fatality rates. A rapid and specific test for RVF diagnosis at the site of a suspected outbreak is crucial for the implementation of control measures. Here, we report the development and the evaluation of the diagnostic performance characteristics of a pen-side test found to be a highly accurate and valuable first-line diagnostic tool for on-site RVF detection.

Mots-clés Agrovoc : diagnostic, immunodiagnostic, virose, transmission des maladies, matériel de laboratoire, surveillance épidémiologique, fièvre de la Vallée du Rift

Mots-clés géographiques Agrovoc : Afrique, Pays arabes

Classification Agris : L73 - Maladies des animaux
U30 - Méthodes de recherche

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

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