Favre Felicien, Jourda Cyril, Grisoni Michel, Besse Pascale, Charron Carine.
2019. Advancements in breeding Fusarium resistant vanilla varieties using Next Generation Sequencing.
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Résumé : Demand for natural vanilla flavor is increasing, but the cultivated Vanilla planifolia faces critical challenges in response to biotic and abiotic stresses. In particular, Root and Stem Rot (RSR) disease caused by Fusarium oxysporum f. sp. radicis-vanillae (Forv) is the most damaging disease of vanilla. What can we do to reduce the damage? Prophylactic measures and use of chemicals or biocontrol agents are not effective in controlling RSR in the field. In this context, the use of resistant varieties is the best option to reduce Fusarium impact. Low genetic diversity has been detected within V. planifolia in accordance with the vegetative mode of dispersion of the vine. However, among the V. planifolia cultivars conserved in the Biological Resources Center Vatel at CIRAD La Réunion, several showed Forv resistance in lab tests as well as in the field. The exploration and deciphering of vanilla genomes using Next Generation Sequencing (NGS) provides a real reservoir of knowledge in support to the vanilla breeding programs. In particular, it opens access to genes involved in RSR plant resistance, allowing the development of molecular markers genetically linked to resistance.
Mots-clés libres : Fusarium oxysporum, Vanilla planifolia, NGS, Plant resistance
Auteurs et affiliations
- Favre Felicien, Université de la Réunion (REU)
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Jourda Cyril, CIRAD-BIOS-UMR PVBMT (REU)
ORCID: 0000-0002-7799-3501
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Grisoni Michel, CIRAD-BIOS-UMR PVBMT (MDG) ORCID: 0000-0001-9867-9098
- Besse Pascale, Université de la Réunion (REU)
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Charron Carine, CIRAD-BIOS-UMR PVBMT (REU) ORCID: 0000-0003-0281-9936
Source : Cirad-Agritrop (https://agritrop.cirad.fr/594418/)
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